Beyond Food Centre of Excellence, AgResearch Ltd, Christchurch, New Zealand.
Riddet Institute, based at Massey University, Palmerston North, New Zealand.
Int J Cosmet Sci. 2022 Feb;44(1):82-90. doi: 10.1111/ics.12756. Epub 2021 Dec 22.
Human hair is regularly subjected to chemical and physical insults, such as heat, UV-irradiation and alkaline hair care products. These insults result in molecular modifications at the hair protein level that underpin mechanical and sensory property changes in the fibres. These changes can manifest itself in reduced hair quality and performance attributes observable to the consumer. In this work, changes in protein modification as a result of heat and alkaline treatments are determined.
Redox proteomic profiling using high-resolution mass spectrometry was applied to map and evaluate amino acid residue modifications in human hair exposed to a combination of thermal treatments and alkali exposure with the aim to understand the underlying chemical processes.
Our results show that an increase in redox-related modifications is associated with exposure to higher levels of hydrothermal and alkaline insult. Post-translational modification profiling at the protein primary structural level delivered some further insights into the site-specificity of these modifications, with a clear increase in the number of cysteic acid modifications noticed in samples subjected to more extreme insults.
Pinpointing modification sides within proteins and the hair shaft proteome can be used as a basis for employing mitigation or repair strategies of hair protein damage caused by environmental or hair treatment-related insults.
人类的头发经常受到化学和物理的刺激，如热、紫外线辐射和碱性护发产品。这些刺激会导致头发蛋白质水平的分子修饰，从而支撑纤维的机械和感官性能的变化。这些变化会导致头发质量和性能属性的降低，消费者可以观察到这些变化。在这项工作中，确定了热和碱性处理导致的蛋白质修饰变化。
使用高分辨率质谱的氧化还原蛋白质组学分析方法，用于绘制和评估暴露于热疗和碱暴露组合的人类头发中氨基酸残基的修饰，旨在了解潜在的化学过程。
我们的结果表明，与更高水平的水热和碱性刺激相比，与氧化还原相关的修饰增加。在蛋白质一级结构水平上的翻译后修饰分析提供了这些修饰的特异性的进一步见解，在经历更极端刺激的样品中，半胱氨酸酸修饰的数量明显增加。
在蛋白质和头发轴蛋白质组内确定修饰侧，可以作为采用减轻或修复环境或头发处理相关刺激引起的头发蛋白质损伤的策略的基础。
