Bernard P A, Max S R
J Neurochem. 1986 Jun;46(6):1942-6. doi: 10.1111/j.1471-4159.1986.tb08517.x.
The number of cytosolic androgen receptors in rat skeletal muscle increases following denervation and disuse. This increase was postulated to represent altered intracellular distribution and consequent diminished sensitivity of skeletal muscle to androgens. To test this hypothesis, we measured total (homogenate) androgen receptor levels after denervation. Total (homogenate) androgen receptor binding did not change in response to denervation of leg muscles from adult male rats. An increase in cytosolic receptor number with no increase in total (homogenate) receptor levels supports the hypothesis of altered intracellular distribution of androgen receptors in denervated muscle. Cytosolic androgen receptor binding in muscle from male rats increased by 40% after denervation, whereas in females the increase was 17%. These increases could not be altered by endocrine manipulations of males or females.
去神经支配和废用后，大鼠骨骼肌中胞质雄激素受体的数量会增加。这种增加被认为代表了细胞内分布的改变以及骨骼肌对雄激素敏感性的相应降低。为了验证这一假设，我们测量了去神经支配后总的（匀浆）雄激素受体水平。成年雄性大鼠腿部肌肉去神经支配后，总的（匀浆）雄激素受体结合没有变化。胞质受体数量增加而总的（匀浆）受体水平没有增加，这支持了去神经支配肌肉中雄激素受体细胞内分布改变的假设。雄性大鼠去神经支配后，肌肉中胞质雄激素受体结合增加了40%，而雌性大鼠增加了17%。这些增加不受雄性或雌性内分泌调节的影响。
