Guangxi University of Chinese Medicine, Guangxi, China.
Guangxi University of Chinese Medicine, Guangxi, China/ Guangxi Advantageous Chinese Medicine and Ethnic Medicine Development Engineering Research Centre, Guangxi, China.
Pak J Pharm Sci. 2021 Sep;34(5(Special)):2009-2020.
Sedum emarginatum Migo (Aoyejingtian) is a perennial succulent herb of the sedum genus in the family Crassulaceae, which has the fountion of treating furuncle, swelling and haematemesis, hematochezia, menorrhagia and hepatitis. Preliminary studies of our research group had showed that the ethyl acetate extract of Sedum emarginatum Migo could inhibit the proliferation of liver cancer HepG2 cells. The establishment of a reasonable and feasible quality evaluation method for the effective parts of Sedum emarginatum Migo can provide a scientific basis for the further development and utilization of Sedum emarginatum Migo. In this study, a multi-wavelength conversion method was used to establish high-performance liquid chromatography (HPLC) fingerprints of the ethyl acetate extract of Sedum emarginatum Migo, and the method was also used to simultaneously determine the gallic acid, protocatechuic acid, caffeic acid, and ferulic acid, isoquercitrin and luteolin in the ethyl acetate extract of Sedum emarginatum Migo. The similarity of the fingerprints of the ethyl acetate extract of Sedum emarginatum Migo from different origins and the content of 6 components were compared. The established method was simple, accurate, table and reliable, which could provide a fast, accurate and reliable method for comprehensive evaluation of the quality of Sedum emarginatum Migo.
景天三七（Aoyejingtian）是景天科景天属的多年生肉质草本植物，具有治疗痈肿、吐血、便血、月经过多和肝炎的作用。本研究组的初步研究表明，景天三七的乙酸乙酯提取物可抑制肝癌 HepG2 细胞的增殖。建立景天三七有效部位合理可行的质量评价方法，可为景天三七的进一步开发利用提供科学依据。本研究采用多波长转换法建立了景天三七乙酸乙酯提取物的高效液相色谱（HPLC）指纹图谱，并采用该方法同时测定了景天三七乙酸乙酯提取物中没食子酸、原儿茶酸、咖啡酸、阿魏酸、异槲皮苷和木樨草素。比较了不同来源景天三七乙酸乙酯提取物的指纹图谱相似度和 6 种成分的含量。建立的方法简单、准确、快速、可靠，可为景天三七的质量综合评价提供快速、准确、可靠的方法。
