Department of Food Material Science, Institute of Food Science and Biotechnology, University of Hohenheim, Garbenstrasse 21/25, 70599 Stuttgart, Germany.
German Institute for Food Technology, Product Innovation, Professor-von-Klitzing-Straße 7, 49610 Quakenbrück, Germany.
Food Res Int. 2021 Dec;150(Pt B):110803. doi: 10.1016/j.foodres.2021.110803. Epub 2021 Nov 8.
There is an increasing demand to develop and characterize high moisture extrudates from alternative plant proteins due to their increased use in various foods. In this study, wet texturized proteins from two pea isolates and four oilseed flours from pumpkin and sunflower were subjected to an acid titration to gain insights into their buffering capacity. Results were compared to pork meat with a special emphasis on compositional differences. Wet texturized pumpkin and sunflower proteins had the highest buffering capacity, especially in between pH7.0 and pH4.5, while pea protein extrudates and pork meat were more prone to acidification and similar in buffering capacity. A multiple linear regression model further revealed that ash and select minerals and amino acids are key influencing factors on the overall buffering capacity, while the effect of protein and non-protein nitrogen depends on the evaluated pH-regime. The obtained results underline the importance for a more in-depth physicochemical characterization of texturized plant proteins and their raw materials and suggest a need for recipe and process adjustment to achieve stable pH values.
由于替代植物蛋白在各种食品中的应用越来越多,人们对开发和表征高水分挤出物的需求也在不断增加。在这项研究中,对两种豌豆分离蛋白和四种来自南瓜和向日葵的油料面粉的湿组织化蛋白进行了酸滴定,以深入了解它们的缓冲能力。研究结果与猪肉进行了比较,特别强调了它们在组成上的差异。湿组织化的南瓜和向日葵蛋白具有最高的缓冲能力,特别是在 pH7.0 和 pH4.5 之间,而豌豆蛋白挤出物和猪肉更容易酸化,缓冲能力相似。多元线性回归模型进一步表明,灰分和一些特定的矿物质和氨基酸是整体缓冲能力的关键影响因素,而蛋白质和非蛋白质氮的影响则取决于所评估的 pH 范围。研究结果强调了对组织化植物蛋白及其原料进行更深入的物理化学特性分析的重要性,并表明需要调整配方和工艺以实现稳定的 pH 值。