Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, PR China; Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, PR China.
Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, PR China; Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, PR China; Engineering Research Center for Nanophotonics and Advanced Instrument, Ministry of Education, East China Normal University, Shanghai 200062, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, PR China.
J Hazard Mater. 2022 Apr 5;427:127915. doi: 10.1016/j.jhazmat.2021.127915. Epub 2021 Nov 27.
Food waste (FW) is important object of resource utilization and source of antibiotic resistance genes (ARGs). This study investigated the effects of biodrying combined with inoculating mature compost (B&M) on the composting efficiency, succession of bacterial communities and their links with metabolism functions as well as the fate of ARGs during FW composting. The results showed that B&M could rapidly raise and maintain high relative abundance of Bacillaceae (66.59-94.44%) as well as composting temperature (45.86-65.86 ℃), so as to achieve the final maturity of FW composting in a short time by regulating microbial carbohydrate (14.02-15.31%) and amino acid metabolism (10.33-12.47%). Network analysis demonstrated that high temperature could effectively inhibit the proliferation and spread of potential bacterial hosts of ARGs and integrons including Lactobacillaceae, Enterobacteriaceae, Leuconostocaceae and Corynebacteriaceae during the first two days of composting. As a result, B&M significantly reduced the absolute (72.09-99.47%) and relative abundances (0.31-2.44 logs) of nearly all ARGs especially ermB, tetM, bla and bla. Present study deepened the knowledge of ARGs variation, succession and metabolism functions of bacterial communities when B&M processes were used for FW composting, suggesting a promising technology for reducing the transmission risk of ARGs and reaching maturity of FW composting.
食物垃圾（FW）是资源利用的重要对象和抗生素耐药基因（ARGs）的来源。本研究调查了生物干燥结合接种成熟堆肥（B＆M）对堆肥效率、细菌群落演替及其与代谢功能的关系以及 FW 堆肥过程中 ARGs 命运的影响。结果表明，B＆M 可以快速提高并维持芽孢杆菌科（66.59-94.44％）以及堆肥温度（45.86-65.86℃）的相对丰度，从而通过调节微生物碳水化合物（14.02-15.31％）和氨基酸代谢（10.33-12.47％）在短时间内实现 FW 堆肥的最终成熟。网络分析表明，高温可在堆肥的头两天有效抑制 ARGs 和整合子的潜在细菌宿主（包括乳杆菌科、肠杆菌科、明串珠菌科和棒状杆菌科）的增殖和传播。因此，B＆M 显著降低了几乎所有 ARGs（特别是 ermB、tetM、bla 和 bla）的绝对丰度（72.09-99.47％）和相对丰度（0.31-2.44 log）。本研究深化了 B＆M 处理 FW 堆肥时 ARGs 变化、细菌群落演替和代谢功能的知识，为降低 ARGs 传播风险和实现 FW 堆肥成熟提供了一种有前途的技术。
