Huang Xiao, Duan Chongsen, Yu Jianghua, Dong Wenyi
Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing 210044, China; Shenzhen Key Laboratory of Water Resources Utilization and Environmental Pollution Control, School of Civil and Environmental Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China.
Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing 210044, China.
Bioresour Technol. 2022 Feb;345:126471. doi: 10.1016/j.biortech.2021.126471. Epub 2021 Dec 2.
For investigating the microbial community, interspecific interaction and nitrogen metabolism during the transform process from heterotrophic to synergistic and autotrophic denitrification, a filter was built, and carbon source and sulfur concentration were changed to release the transformation process. The results demonstrated that the transformation process was feasible to keep nitrate nitrogen (NO-N) discharge concentration lower than 15 mg L, however, nitrite nitrogen (NO-N) accumulation and its rate reached 7.85% at initial stages. The dominant denitrification gunes were Methylophilaceae, Thiovulaceae and Hydrogenophilaceae for three processes, respectively, and the microbial interspecific interaction of heterotrophic denitrification was more complex than others. NO-N accumulation was confirmed by the low abundance of EC1.7.7.1 and EC1.7.2.1, and the dominance degree of dark oxidation of sulfur compounds and dark sulfide oxidation improved in synthesis and autotrophic denitrifications.
为研究从异养反硝化到协同反硝化和自养反硝化转变过程中的微生物群落、种间相互作用和氮代谢，构建了一个滤池，并改变碳源和硫浓度以释放转变过程。结果表明，将硝酸盐氮（NO-N）排放浓度保持在15 mg/L以下的转变过程是可行的，然而，在初始阶段亚硝酸盐氮（NO-N）积累及其速率达到7.85%。三个过程中主要的反硝化菌分别为嗜甲基菌科、硫氧化菌科和嗜氢菌科，异养反硝化的微生物种间相互作用比其他过程更复杂。EC1.7.7.1和EC1.7.2.1的低丰度证实了NO-N的积累，并且在合成反硝化和自养反硝化中，硫化合物暗氧化和暗硫化物氧化的优势程度有所提高。
