Tocco-Bradley R, Moldawer L L, Jones C T, Gerson B, Blackburn G L, Bistrian B R
Proc Soc Exp Biol Med. 1986 Jun;182(2):263-71. doi: 10.3181/00379727-182-42338.
The present study summarizes the biological response of rats to infusion with recombinant murine IL-1 (rIL-1) cloned in Escherichia coli. Thirty-seven male rats (135-180 g) were infused over a 6-hr period with either 0.008 M guanidine hydrochloride (the vehicle) or E. coli product (both groups are controls) or 1000, 3750, 7500, 15,000, or 37,500 LAF units/hr of rIL-1. The controls and the group receiving 1000 LAF units/hr of rIL-1 did not exhibit a change in body temperature during the experiment. A mild fever was noted with 3750 LAF units/hr which became significantly elevated with 7500 and 15,000 LAF units/hr. At a dose of 37,500 LAF units/hr of rIL-1 (in 0.08 M guanidine hydrochloride) the rats became hypothermic and died. An equivalent dose of guanidine hydrochloride alone (0.08 M) was not fatally toxic although the rats did become hypothermic. Plasma zinc levels were significantly depressed and white blood cell count elevated at 6 hr postinfusion onset. Resting energy expenditure (REE) was significantly depressed during an infusion of 7500 and 15,000 LAF units/hr of rIL-1 despite a concurrent elevation in body temperature. Whole-body leucine kinetics were unchanged by infusion with rIL-1. Plasma fibrinogen and serum haptoglobin and copper levels were not altered by rIL-1. In conclusion, murine rIL-1 is similar to monocytic-derived IL-1 in that it produces a fever, hypozincemia, and leukocytosis; however, rIL-1 does not induce changes in protein metabolism.
本研究总结了大鼠对输注在大肠杆菌中克隆的重组鼠白细胞介素-1（rIL-1）的生物学反应。37只雄性大鼠（体重135 - 180克）在6小时内分别输注0.008M盐酸胍（溶媒）或大肠杆菌产物（两组均为对照）或1000、3750、7500、15000或37500LAF单位/小时的rIL-1。在实验过程中，对照组和接受1000LAF单位/小时rIL-1的组体温未出现变化。输注3750LAF单位/小时时出现轻度发热，输注7500和15000LAF单位/小时时体温显著升高。当rIL-1剂量为37500LAF单位/小时（溶于0.08M盐酸胍中）时，大鼠体温过低并死亡。单独使用等量的盐酸胍（0.08M）虽不会致命，但大鼠会出现体温过低。输注开始后6小时，血浆锌水平显著降低，白细胞计数升高。在输注7500和15000LAF单位/小时rIL-1期间，尽管体温同时升高，但静息能量消耗（REE）显著降低。输注rIL-1对全身亮氨酸动力学无影响。rIL-1未改变血浆纤维蛋白原、血清触珠蛋白和铜水平。总之，鼠rIL-1与单核细胞来源的IL-1相似，都会引起发热、低锌血症和白细胞增多；然而，rIL-1不会诱导蛋白质代谢发生变化。
