SATIE UMR CNRS 8029, CY Cergy Paris University, Cergy-Pontoise, 95000, France.
SATIE UMR CNRS 8029, CY Cergy Paris University, Cergy-Pontoise, 95000, France.
Biosens Bioelectron. 2022 Feb 15;198:113831. doi: 10.1016/j.bios.2021.113831. Epub 2021 Nov 27.
As an answer to alternative non-animal testing, biosensors dedicated to the ex vivo skin explants monitoring are a challenge to study physiological-like behavior and optimize new topical products. Because of the skin viscoelastic behavior, mechanical tests are commonly based on macroscopic measurement and give global descriptors of its state. Other techniques, including photoacoustic ones, are more focused on the molecular scale. There is a gap to fill in the mesoscopic range to get information about the microstructure of the skin. This article presents the proof-of-concept of a biosensor coupling a thickness shear-mode transducer with human skin explants kept in life-like state for a week. Thanks to a multifrequency analysis of the transducer impedance, this biosensor is able to monitor the viscoelastic properties of the skin. To extract the complex shear modulus and the microstructural evolutions, a mechanical model based on fractional calculus is used. As a preliminary results, the sensitivity of the sensor to probe the skin viscoelasticity in lifelike state and the impact of its culture medium are presented. A suitable microstructural coefficient is also extracted in order to identify mechanical breaches in the skin barrier after the application of peeling products.
作为替代非动物测试的一种方法，专门用于监测离体皮肤样本的生物传感器是研究生理样性行为和优化新型局部产品的一种挑战。由于皮肤的粘弹性行为，机械测试通常基于宏观测量，并提供其状态的整体描述。其他技术，包括光声技术，更侧重于分子尺度。在介观范围内存在一个空白，以获取有关皮肤微观结构的信息。本文提出了一种将厚度剪切模式换能器与保持在类似生命状态下一周的人体皮肤样本耦合的生物传感器的概念验证。通过对换能器阻抗的多频分析，该生物传感器能够监测皮肤的粘弹性特性。为了提取复杂的剪切模量和微观结构演变，使用基于分数微积分的机械模型。作为初步结果，展示了传感器在类似生命状态下探测皮肤粘弹性的灵敏度及其培养基的影响。还提取了合适的微观结构系数，以便在应用剥落产品后识别皮肤屏障的机械破裂。
