Assunção Joana, Amaro Helena M, Lopes Graciliana, Tavares Tânia, Malcata F Xavier, Guedes A Catarina
CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, Novo Edíficio do Terminal de Cruzeiros do Porto de Leixões, Matosinhos, Portugal.
LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, University of Porto, Porto, Portugal.
J Appl Microbiol. 2022 Apr;132(4):2844-2858. doi: 10.1111/jam.15403. Epub 2021 Dec 28.
The major aim of this work was to consistently optimize the production of biomass of the dinoflagellate Karlodinium veneficum and evaluate its extracts biotechnological potential application towards food, nutraceutical or/and pharmaceutical industries.
A successful approach of biomass production of K. veneficum CCMP 2936 was optimized along with the chemical characterization of its metabolite profile. Several temperatures (12, 16, 20, 25, 30°C), L1 nutrient concentrations (0.5×, 2×, 2.5×, 3×) and NaCl concentrations (20, 25, 30, 40 g L ) were tested. The growth rate was maximum at 16°C, 2× nutrient concentration and 40 g L of NaCl; hence, these conditions were chosen for bulk production of biomass. Methanolic extracts were prepared, and pigments, lipids and phenolic compounds were assessed; complemented by antioxidant and anti-inflammatory capacities, and cytotoxicity. Fucoxanthin and derivatives accounted for 0.06% of dry weight, and up to 60% (w/w) of all quantified metabolites were lipids. Said extracts displayed high antioxidant capacity, as towards assessed via the NO and ABTS assays (IC = 109.09 ± 6.73 and 266.46 ± 2.25 µg ml , respectively), unlike observed via the O assay (IC reaching 56.06 ± 5.56 µg ml ). No signs of cytotoxicity were observed.
Karlodinium veneficum biomass production was consistently optimized in terms of temperature, L1 nutrient concentrations and NaCl concentration. In addition, this strain appears promising for eventual biotechnological exploitation.
This work provides fundamental insights about the growth and potential of value-added compounds of dinoflagellate K. veneficum. Dinoflagellates, as K. veneficum are poorly studied regarding its biomass production and added-value compounds for potential biotechnological exploitation. These organisms are difficult to maintain and grow in the laboratory. Thus, any fundamental contribution is relevant to share with the scientific community.
本工作的主要目的是持续优化有毒卡尔藻的生物量生产，并评估其提取物在食品、营养保健品或/和制药行业的生物技术潜在应用。
优化了有毒卡尔藻CCMP 2936生物量生产的成功方法，并对其代谢产物谱进行了化学表征。测试了几种温度（12、16、20、25、30°C）、L1营养浓度（0.5×、2×、2.5×、3×）和NaCl浓度（20、25、30、40 g/L）。在16°C、2倍营养浓度和40 g/L NaCl条件下生长速率最高；因此，选择这些条件进行生物量的大规模生产。制备了甲醇提取物，并评估了色素、脂质和酚类化合物；辅以抗氧化和抗炎能力以及细胞毒性。岩藻黄质及其衍生物占干重的0.06%，所有定量代谢产物中高达60%（w/w）为脂质。所述提取物表现出高抗氧化能力，通过NO和ABTS测定法评估（IC50分别为109.09±6.73和266.46±2.25 μg/ml），与通过O2测定法观察到的情况不同（IC50达到56.06±5.56 μg/ml）。未观察到细胞毒性迹象。
在温度、L1营养浓度和NaCl浓度方面，有毒卡尔藻的生物量生产得到了持续优化。此外，该菌株在最终的生物技术开发方面似乎很有前景。
这项工作提供了关于有毒卡尔藻生长和增值化合物潜力的基本见解。像有毒卡尔藻这样的甲藻，在其生物量生产和用于潜在生物技术开发的增值化合物方面研究较少。这些生物在实验室中难以维持和生长。因此，任何基础性贡献都与科学界分享相关。
