Rich S A, Owens T R, Anzola M C, Bartholomew L E
Arthritis Rheum. 1986 Apr;29(4):501-7. doi: 10.1002/art.1780290407.
A sensitive in vitro bioassay for the alpha-interferon induction of lupus-type inclusions (LI) has been established with the human B lymphoblastoid cell line, Daudi. Sera from 11 patients with systemic lupus erythematosus (SLE) were evaluated with this assay. LI induction by these sera increased in proportion to their antiviral activity on Madin-Darby bovine kidney (MDBK) cells. Two of these sera did not induce LI; they showed no antiviral activity on the MDBK cell assay. Clinically and serologically, their donors were in remission. Two sera induced the formation of LI that exceeded the maximum frequencies obtained with 3 alpha-interferon preparations. These sera had the greatest antiviral activities, and their donors had the greatest disease activities. Antisera to alpha-interferons prevented the induction of LI with the pure and homogeneous recombinant human leukocyte interferon, IFLrA, and SLE sera. Together, these results provide evidence that the alpha-interferon endogenous to SLE patients has a great ability to induce LI, and the SLE serum induction of LI corresponds well to the patient's disease activity.
利用人B淋巴母细胞系Daudi建立了一种用于检测α干扰素诱导狼疮样包涵体（LI）的灵敏体外生物测定法。用该测定法评估了11例系统性红斑狼疮（SLE）患者的血清。这些血清诱导LI的能力与其对Madin-Darby牛肾（MDBK）细胞的抗病毒活性成比例增加。其中两份血清未诱导LI形成；它们在MDBK细胞测定中未显示抗病毒活性。从临床和血清学角度来看，其供血者处于缓解期。两份血清诱导LI形成的频率超过了3种α干扰素制剂所获得的最高频率。这些血清具有最强的抗病毒活性，其供血者的疾病活动度也最高。抗α干扰素血清可阻止用纯的、均一的重组人白细胞干扰素IFLrA和SLE血清诱导LI形成。这些结果共同表明，SLE患者内源性α干扰素具有很强的诱导LI的能力，SLE血清诱导LI的情况与患者的疾病活动度密切相关。
