Malinski J A, Nelsestuen G L
Biochim Biophys Acta. 1986 Jun 19;882(2):177-82. doi: 10.1016/0304-4165(86)90152-2.
The process of platelet aggregation as detected by turbidity changes in the platelet aggregometer was studied relative to light scattering by large particles. For latex beads a plot of light scattering intensity/unit mass versus particle size gave increased light scattering intensity for small particle sizes but decreased scattering at large particle size. This behavior is predicted by Rayleigh-Gans theory. These results were related to the platelet aggregometer, an optical instrument used to measure the association of small particles (monomeric platelets) to large particles (platelet aggregates). Formalin-fixed platelets do not show changes in light transmission due to energy-requiring processes, such as shape change, so that turbidity changes in the presence of aggregating agents could be attributed to a change in platelet aggregation state. Small platelet aggregates showed increased turbidity compared to a similar mass of monomeric platelets. In fact, very large platelet aggregates that were visible to the unaided eye were needed to produce a decrease in light scattering intensity. Thus, turbidity can either increase or decrease with platelet aggregation depending on the size of the aggregates. Studies of platelet aggregation that show no initial increase in turbidity must be characterized by dominance of large platelet aggregates and monomeric platelets.
通过血小板聚集仪中浊度变化检测的血小板聚集过程，相对于大颗粒的光散射进行了研究。对于乳胶珠，光散射强度/单位质量与粒径的关系图显示，小粒径时光散射强度增加，而大粒径时散射减少。这种行为由瑞利 - 甘斯理论预测。这些结果与血小板聚集仪相关，血小板聚集仪是一种用于测量小颗粒（单体血小板）与大颗粒（血小板聚集体）结合的光学仪器。福尔马林固定的血小板不会因需要能量的过程（如形状变化）而显示透光率变化，因此聚集剂存在时的浊度变化可归因于血小板聚集状态的改变。与相同质量的单体血小板相比，小血小板聚集体显示出更高的浊度。事实上，需要肉眼可见的非常大的血小板聚集体才能使光散射强度降低。因此，浊度会随着血小板聚集而增加或减少，这取决于聚集体的大小。那些显示浊度无初始增加的血小板聚集研究，其特征必定是大血小板聚集体和单体血小板占主导。
