Li Jiaqi, Sun Xiaoxiao, Luo Shuoming, Lin Jian, Xiao Yang, Yu Haibo, Huang Gan, Li Xia, Xie Zhiguo, Zhou Zhiguang
National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology (Central South University), Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China.
Front Pharmacol. 2021 Nov 11;12:729890. doi: 10.3389/fphar.2021.729890. eCollection 2021.
To investigate the association between susceptibility to type 1 diabetes mellitus (T1DM) and polymorphisms (rs1143627 and rs1143643) in the interleukin 1 beta (IL1B) gene in the Chinese Han population. The Meso Scale Discovery (MSD) method was used to detect the concentration of IL-1β in 24 T1DM patients and 27 healthy controls. MassARRAY was used to analyze the polymorphisms in the IL1B gene in 510 patients with classic T1DM and 531 healthy controls. The general data of the T1DM patients and healthy controls were compared by the chi-square test and Mann-Whitney U test. The chi-square test and logistic regression were used to analyze the frequency distributions of alleles and genotypes of polymorphisms in the IL1B gene. The Kruskal-Wallis H test and chi-square test were used for the genotype-phenotype analysis of rs1143627 and rs1143643 in the IL1B gene. ① The concentration of IL-1β in T1DM patients was significantly higher than that in healthy controls. ② rs1143627 and rs1143643 in the IL1B gene were significantly correlated with the positivity rates for IA-2A and ZnT8A; genotype GG at rs1143627 and genotype CC at rs1143643 in the case group showed lower positivity rates for IA-2A and ZnT8A. ③ There was no significant difference in the genotypes or allele frequencies at rs1143627 (GG/GA/AA) or rs1143643 (CC/CT/TT) between the case group and control group ( > 0.05). ④ rs1143627 and rs1143643 were not found to be linked to T1DM susceptibility under different genetic models. rs1143627 and rs1143643 in the IL1B gene correlate with the positivity rate of IA-2A and ZnT8A in Chinese Han individuals with T1DM
为研究中国汉族人群中1型糖尿病（T1DM）易感性与白细胞介素1β（IL1B）基因多态性（rs1143627和rs1143643）之间的关联。采用Meso Scale Discovery（MSD）方法检测24例T1DM患者和27例健康对照者血清IL-1β浓度。运用MassARRAY技术分析510例经典T1DM患者和531例健康对照者IL1B基因多态性。采用卡方检验和Mann-Whitney U检验比较T1DM患者和健康对照者的一般资料。运用卡方检验和logistic回归分析IL1B基因多态性的等位基因和基因型频率分布。采用Kruskal-Wallis H检验和卡方检验对IL1B基因rs1143627和rs1143643进行基因型-表型分析。①T1DM患者血清IL-1β浓度显著高于健康对照者。②IL1B基因rs1143627和rs1143643与IA-2A和ZnT8A阳性率显著相关；病例组中rs1143627位点的GG基因型和rs1143643位点的CC基因型的IA-2A和ZnT8A阳性率较低。③病例组与对照组在rs1143627（GG/GA/AA）或rs1143643（CC/CT/TT）的基因型或等位基因频率上无显著差异（>0.05）。④在不同遗传模型下，未发现rs1143627和rs1143643与T1DM易感性相关。IL1B基因中的rs1143627和rs1143643与中国汉族T1DM患者IA-2A和ZnT8A的阳性率相关
