Kumar Santosh, Bharti Daizy, Shazib Shahed Uddin Ahmed, Shin Mann Kyoon
Department of Biological Sciences, College of Natural Sciences, University of Ulsan, Ulsan, South Korea.
Zoological Survey of India, Kolkata, India.
Front Microbiol. 2021 Nov 19;12:761961. doi: 10.3389/fmicb.2021.761961. eCollection 2021.
Very few studies exist on the description of protozoan ciliates from industrially contaminated sites. In this study, we report a description of a novel hypotrich ciliate isolated from water samples collected from an industrially contaminated outlet in Onsan, Ulsan, South Korea. The oxytrichid ciliate, n. sp., was investigated using live observation and protargol impregnation. The morphology, morphogenesis, and molecular phylogeny inferred from small-subunit (SSU) rRNA gene sequences were studied. The new species is mainly characterized by a cell size of about 70 × 40 μm , two elongate ellipsoidal macronuclear nodules and one or two micronuclei, adoral zone of about 51% of body length with 32 membranelles on average, about 34 cirri in the right and 24 cirri in the left marginal row, 18 frontoventral transverse cirri, six dorsal kineties including two dorsomarginal rows, and dorsal kinety 1 with 26 bristles. Morphogenesis is similar to that of the type species, i.e., , except that oral primordium does not contribute to anlage II of the proter. Phylogenetic analyses, based on small-subunit rRNA gene sequences, consistently place the new species within the family Oxytrichidae, clustering with .
关于来自工业污染场地的原生动物纤毛虫的描述的研究非常少。在本研究中，我们报告了一种从韩国蔚山温山一个工业污染排放口采集的水样中分离出的新型腹毛目纤毛虫的描述。使用活体观察和蛋白银染色法对这种尖毛科纤毛虫新物种进行了研究。研究了从小亚基（SSU）rRNA基因序列推断出的形态、形态发生和分子系统发育。新物种的主要特征是细胞大小约为70×40μm，有两个细长椭圆形的大核结节和一或两个小核，口围带约占体长的51%，平均有32个小膜，右侧边缘列约有34根纤毛，左侧边缘列有24根纤毛，额腹横纤毛18根，包括两列背边缘列在内的6列背纤毛，以及背纤毛列1有26根刚毛。形态发生与模式种相似，即 ，不同之处在于口原基对前仔虫的第二原基没有贡献。基于小亚基rRNA基因序列的系统发育分析一致将新物种置于尖毛科内，与 聚类。
