Whitney P A, Morris D R
J Bacteriol. 1978 Apr;134(1):214-20. doi: 10.1128/jb.134.1.214-220.1978.
Strains of yeast have been constructed that are unable to synthesize ornithine and are thereby deficient in polyamine biosynthesis. These strains were used to develop a protocol for isolation of mutants blocked directly in polyamine synthesis. There were seven mutants isolated that lack ornithine decarboxylase activity; these strains exhibited greatly decreased pool levels of putrescine, spermidine, and spermine when grown in the absence of polyamines. Three of the mutants lack S-adenosylmethionine decarboxylase activity; polyamine limitation of a representative mutant resulted in an accumulation of putrescine and a decrease in spermidine and spermine. When the mutants were cultured in the absence of polyamines, a continuously declining growth rate was observed.
已经构建了不能合成鸟氨酸从而在多胺生物合成方面存在缺陷的酵母菌株。这些菌株被用于开发一种分离直接在多胺合成中受阻的突变体的方案。分离出了七个缺乏鸟氨酸脱羧酶活性的突变体；当在没有多胺的情况下生长时，这些菌株中腐胺、亚精胺和精胺的库水平大大降低。其中三个突变体缺乏S-腺苷甲硫氨酸脱羧酶活性；对一个代表性突变体进行多胺限制导致腐胺积累，亚精胺和精胺减少。当在没有多胺的情况下培养这些突变体时，观察到生长速率持续下降。
