McCarthy J E, Sebald W, Gross G, Lammers R
Gene. 1986;41(2-3):201-6. doi: 10.1016/0378-1119(86)90099-5.
The cDNA sequences encoding mature human interleukin 2 (IL2) and beta-interferon (INF beta), respectively, were fused with various translational initiation regions and inserted into two different types of expression vector. The relative levels of expression of the two genes and the functional stability of their respective mRNAs were examined in vivo in Escherichia coli hosts. The addition of the 30-bp sequence, found immediately upstream of the E. coli atpE gene Shine-Dalgarno (SD) sequence, to the translational initiation regions of IL2 and INF beta increased the expression of both these genes by a factor of 6-10. Thus this sequence, which naturally acts within the E. coli atp operon to enhance the translational initiation frequency of the atpE gene, can increase the expression of other genes in E. coli. It may exemplify a specific type of recognition signal for the E. coli translational apparatus.
分别编码成熟人白细胞介素2（IL2）和β干扰素（INFβ）的cDNA序列与各种翻译起始区域融合，并插入两种不同类型的表达载体中。在大肠杆菌宿主中体内检测了这两个基因的相对表达水平及其各自mRNA的功能稳定性。将在大肠杆菌atpE基因的Shine-Dalgarno（SD）序列上游紧邻处发现的30bp序列添加到IL2和INFβ的翻译起始区域，使这两个基因的表达增加了6至10倍。因此，该序列在大肠杆菌atp操纵子内自然发挥作用以提高atpE基因的翻译起始频率，它可以增加大肠杆菌中其他基因的表达。它可能例证了大肠杆菌翻译装置的一种特定类型的识别信号。
