Medical Microbiology Laboratory, Department of Microbiology, Center for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli, India.
Microbial Biotechnology Laboratory, Department of Marine Biotechnology, School of Marine Sciences, Bharathidasan University, Tiruchirappalli, India.
Front Immunol. 2021 Nov 16;12:764662. doi: 10.3389/fimmu.2021.764662. eCollection 2021.
is a severe aquaculture pathogen that can infect many hosts including humans, animals, and fish. Timely diagnosis and treatment are crucial for the control of edwardsiellosis in the aqua industry. By using rabbit polyclonal antibody, an expression gene library of virulent strain ED-BDU 1 isolated in south India was constructed and screened. The identified immune expressive proteins were characterized, and the corresponding coding sequences were cloned, expressed, and the purified recombinant proteins were used as antigens. The identified immunoreactive proteins namely HflC, HflK, and YhcI were studied for their immune protective potential by challenge experiments. The protective efficacy of HflC, HflK, and YhcI showed that the clearance of from the host with ~ 60% survivability. Further, the immunoreactive proteins induce a strong immune response upon infection and elicit the significant production of IL-10, IFN-γ, Th1, and Th2 mediated mRNA expression and were therefore effective in vaccine production for edwardsiellosis.
是一种严重的水产养殖病原体，可感染包括人类、动物和鱼类在内的许多宿主。及时诊断和治疗对于水产养殖业中控制爱德华氏菌病至关重要。本研究利用兔多克隆抗体，构建并筛选了从印度南部分离的强毒菌株 ED-BDU 1 的表达基因文库。对鉴定出的免疫表达蛋白进行了特征分析，并克隆了相应的编码序列，将纯化的重组蛋白作为抗原。通过攻毒实验研究了鉴定出的免疫反应性蛋白 HflC、HflK 和 YhcI 的免疫保护潜力。HflC、HflK 和 YhcI 的保护效力表明，从宿主中清除的存活率约为 60%。此外，免疫反应蛋白在感染时会引发强烈的免疫反应，并引起 IL-10、IFN-γ、Th1 和 Th2 介导的 mRNA 表达的显著产生，因此在爱德华氏菌病疫苗生产中是有效的。
