Hu Hailiang, Guo Zhenhao, Yang Junjie, Cui Jiebing, Zhang Yingting, Xu Jin
Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, China.
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
Front Plant Sci. 2021 Nov 18;12:751771. doi: 10.3389/fpls.2021.751771. eCollection 2021.
Hooibrenk is an important fast-growing coniferous timber species that is widely used in landscaping. Recently, research on timber quality has gained substantial attention in the field of tree breeding. Wood is the secondary xylem formed by the continuous inward division and differentiation of the vascular cambium; therefore, the development of the vascular cambium is particularly important for wood quality. In this study, we analyzed the transcriptomes of the cambial zone in during different developmental stages using Illumina HiSeq sequencing, focusing on general transcriptome and microRNA (miRNA) data. We performed functional annotation of the differentially expressed genes (DEGs) in the different stages identified by transcriptome sequencing and generated 15 miRNA libraries yielding 4.73 Gb of clean reads. The most common length of the filtered miRNAs was 21nt, accounting for 33.1% of the total filtered reads. We annotated a total of 32 known miRNA families. Some miRNAs played roles in hormone signal transduction (miR159, miR160, and miR166), growth and development (miR166 and miR396), and the coercion response (miR394 and miR395), and degradome sequencing showed potential cleavage sites between miRNAs and target genes. Differential expression of miRNAs and target genes and functional validation of the obtained transcriptome and miRNA data provide a theoretical basis for further elucidating the molecular mechanisms of cellular growth and differentiation, as well as wood formation in the vascular cambium, which will help improve the wood quality of .
霍伊布伦克是一种重要的速生针叶木材树种，广泛用于园林绿化。最近，木材质量研究在树木育种领域受到了广泛关注。木材是维管形成层不断向内分裂和分化形成的次生木质部；因此，维管形成层的发育对木材质量尤为重要。在本研究中，我们使用Illumina HiSeq测序分析了不同发育阶段形成层区域的转录组，重点关注一般转录组和微小RNA（miRNA）数据。我们对转录组测序确定的不同阶段差异表达基因（DEG）进行了功能注释，并生成了15个miRNA文库，产生了4.73 Gb的clean reads。过滤后的miRNA最常见长度为21nt，占总过滤reads的33.1%。我们总共注释了32个已知的miRNA家族。一些miRNA在激素信号转导（miR159、miR160和miR166）、生长发育（miR166和miR396）以及胁迫反应（miR394和miR395）中发挥作用，降解组测序显示了miRNA与靶基因之间的潜在切割位点。miRNA和靶基因的差异表达以及所获得转录组和miRNA数据的功能验证为进一步阐明细胞生长和分化以及维管形成层中木材形成的分子机制提供了理论基础，这将有助于提高[树种名称未明确]的木材质量。
