Ali Muhammad Kazim, Sun Zheng-Hai, Yang Xiao-Meng, Pu Xiao-Ying, Duan Cheng-Li, Li Xia, Wang Lu-Xiang, Yang Jia-Zhen, Zeng Ya-Wen
Biotechnology and Germplasm Resource Institute, Yunnan Academy of Agricultural Sciences, Kunming, China.
Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
Front Genet. 2021 Nov 18;12:789645. doi: 10.3389/fgene.2021.789645. eCollection 2021.
Chilling stress at booting stage can cause floret deterioration and sterility by limiting the supply of food chain and the accumulation of essential mineral elements resulting in reduction of yield and grain quality attributes in rice. Genomic selection of chilling tolerant rice with reference to the accumulation of mineral elements will have great potential to cope with malnutrition and food security in times of climate change. Therefore, a study was conducted to explore the genomic determinants of cold tolerance and mineral elements content in near-isogenic lines (NILs) of rice subjected to chilling stress at flowering stage. Detailed morphological analysis followed by quantitative analysis of 17 mineral elements revealed that the content of phosphorus (P, 3,253 mg/kg) and potassium (K, 2,485 mg/kg) were highest while strontium (Sr, 0.26 mg/kg) and boron (B, 0.34 mg/kg) were lowest among the mineral elements. The correlation analysis revealed extremely positive correlation of phosphorus (P) and copper (Cu) with most of the cold tolerance traits. Among all the effective ear and the second leaf length correlation was significant with half of the mineral elements. As a result of comparative analysis, some QTLs (, , , , and ) identified for calcium (Ca), zinc (Zn), chromium (Cr) and magnesium (Mg) on chromosome number 1, 2, and 6 while, a novel QTL () was identified on chromosome number 1 for P element only. These findings provided bases for the identification of candidate genes involved in mineral accumulation and cold tolerance in rice at booting stage.
孕穗期的低温胁迫会限制食物链供应和必需矿质元素的积累，从而导致小花退化和不育，进而降低水稻产量和谷粒品质属性。参照矿质元素积累情况对耐冷水稻进行基因组选择，在应对气候变化时期的营养不良和粮食安全方面具有巨大潜力。因此，开展了一项研究，以探究在开花期遭受低温胁迫的水稻近等基因系（NILs）中耐冷性和矿质元素含量的基因组决定因素。详细的形态学分析以及对17种矿质元素的定量分析表明，在这些矿质元素中，磷（P，3253毫克/千克）和钾（K，2485毫克/千克）的含量最高，而锶（Sr，0.26毫克/千克）和硼（B，0.34毫克/千克）的含量最低。相关性分析显示，磷（P）和铜（Cu）与大多数耐冷性状呈极显著正相关。在所有有效穗和第二叶长度中，有一半的矿质元素与之相关性显著。比较分析的结果是，在第1、2和6号染色体上鉴定出了一些与钙（Ca）、锌（Zn）、铬（Cr）和镁（Mg）相关的QTL（、、、、和），而仅在第1号染色体上鉴定出了一个与P元素相关的新QTL（）。这些发现为鉴定孕穗期水稻中参与矿质元素积累和耐冷性的候选基因提供了依据。
