In vitro bioproduction and enhancement of moscatilin from a threatened tropical epiphytic orchid, (Willd.) Kraenzl.

Moscatilin, a bibenzyl derivative (stilbenoid), mostly found in one of the largest genera of Orchidaceae; has many therapeutic benefits. Its function as an anticancer agent has been widely demonstrated through many research investigations. However, the compound has not been produced in vitro to date. The present study highlights the development of cultures viz., seedling generation, callus induction and callus regeneration (transformation of callus into plantlets). These cultures were devised to conserve the threatened tropical epiphytic orchid species, and identify their potential towards moscatilin bioproduction in vitro. Among the three culture platforms, callus-derived plantlets could yield high moscatilin when treated with l-Phenylalanine as a precursor. Tissue differentiation was found to be indispensable for the high production of this polyphenol. These cultures also offer potential commercial benefits as they can serve as appropriate platforms to decode moscatilin biosynthesis and other significant bibenzyl derivatives. Elicitors, such as chitosan, salicylic acid, and methyl jasmonate, were found, causing an enhancement in moscatilin content in the cultures. The seedlings obtained can serve towards ecorestoration and preservation of the studied species. Callogenesis was useful in plantlet regeneration, as callus-derived plantlets could be utilized for the enrichment and commercial scale-up of moscatilin-like chemicals.