Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Ibaraki, Japan.
Front Cell Infect Microbiol. 2021 Nov 19;11:741654. doi: 10.3389/fcimb.2021.741654. eCollection 2021.
The commensal microbiota is involved in a variety of diseases. Our group has noticed that patients with Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN) often present with persistent inflammation of the ocular surface, even in the chronic stage, and that this inflammation is exacerbated by colonization of the mucosa by certain bacteria. However, the changes in the composition of the ocular microbiome in SJS/TEN patients with severe ocular complications (SOCs) remain to be fully investigated. Here, we conducted a cross-sectional study of 46 Japanese subjects comprising 9 healthy control subjects and 37 SJS/TEN patients with SOC. The 16S rRNA-based genetic analyses revealed that the diversity of the ocular microbiome was reduced in SJS/TEN patients with SOC compared with that in healthy control subjects. Principal coordinate analysis based on Bray-Curtis distance at the genus level revealed that the relative composition of the ocular microbiome was different in healthy control subjects and SJS/TEN patients with SOC, and that the SJS/TEN patients with SOC could be divided into four groups based on whether their microbiome was characterized by enrichment of species in genus , , or or by simultaneous enrichment in species in genera , , , , and . Collectively, our findings indicate that enrichment of certain bacteria at the ocular surface could be associated with ocular surface inflammation in SJS/TEN patients with SOC.
共生微生物群参与多种疾病。我们的研究小组注意到，史蒂文斯-约翰逊综合征（SJS）/中毒性表皮坏死松解症（TEN）患者经常出现眼部表面持续炎症，即使在慢性阶段也是如此，并且这种炎症会因某些细菌对粘膜的定植而加剧。然而，SJS/TEN 患者严重眼部并发症（SOC）的眼微生物组组成的变化仍有待充分研究。在这里，我们对 46 名日本受试者进行了一项横断面研究，其中包括 9 名健康对照者和 37 名 SJS/TEN 伴有 SOC 的患者。基于 16S rRNA 的遗传分析表明，与健康对照者相比，SOC 的 SJS/TEN 患者的眼部微生物组多样性降低。基于 Bray-Curtis 距离的主坐标分析在属水平上表明，健康对照者和 SJS/TEN 伴有 SOC 的患者的眼部微生物组组成不同，并且 SOC 的 SJS/TEN 患者可以根据其微生物组是否以属 中物种的富集为特征，或同时以属 中物种的富集为特征， 和 ， 和 ， 和 。总的来说，我们的研究结果表明，眼部表面某些细菌的富集可能与 SJS/TEN 伴有 SOC 的患者的眼部表面炎症有关。
