Jiang Wen-Jie, Liu Wen, Li Ying-Hua, Jiang Hao, Xu Yong-Nan, Kim Nam-Hyung
Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea.
Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Wuyi University, Jiangmen, Guangdong, China.
Toxicon. 2022 Jan 15;205:84-90. doi: 10.1016/j.toxicon.2021.11.124. Epub 2021 Dec 3.
Citrinin (CTN) is a polyketide mycotoxin produced by several strains of Penicillium, Monascus, and Aspergillus. While CTN poses various toxic effects on the female reproductive system in animals, its direct effects on germ cell development are unclear. This study aimed to evaluate the effects of increasing concentrations of CTN (0,20,40,80,100 μM) on porcine oocyte in vitro maturation. Our results indicate that CTN supplementation inhibited polar body extrusion in a dose-dependent manner. Actin and spindle assembly were also disrupted after treatment, indicating that CTN affects the cytoskeleton of porcine oocytes. Oxidative stress and apoptosis were observed under CTN treatment to explore the cause of meiotic maturation failure in porcine oocytes. The results showed that reactive oxygen species levels, cathepsin B activity, and caspase-3 activity were increased in the treated group, indicating that CTN induced oxidative stress and apoptosis. In conclusion, CTN exposure could reduce porcine oocyte maturation by affecting cytoskeletal dynamics, oxidative stress, and apoptosis.
桔霉素（CTN）是一种由多种青霉菌、红曲霉菌和曲霉菌株产生的聚酮类霉菌毒素。虽然CTN对动物的雌性生殖系统有多种毒性作用，但其对生殖细胞发育的直接影响尚不清楚。本研究旨在评估不同浓度（0、20、40、80、100 μM）的CTN对猪卵母细胞体外成熟的影响。我们的结果表明，添加CTN以剂量依赖的方式抑制了极体排出。处理后肌动蛋白和纺锤体组装也受到破坏，表明CTN影响猪卵母细胞的细胞骨架。在CTN处理下观察氧化应激和细胞凋亡，以探究猪卵母细胞减数分裂成熟失败的原因。结果显示，处理组的活性氧水平、组织蛋白酶B活性和半胱天冬酶-3活性均升高，表明CTN诱导了氧化应激和细胞凋亡。总之，接触CTN可通过影响细胞骨架动力学、氧化应激和细胞凋亡来降低猪卵母细胞的成熟率。
