RNA 干扰抑制 FUBP1 基因表达对胃癌细胞系 SGC7901 生物学功能的影响。
Effect of RNA Interference Inhibiting the Expression of the FUBP1 Gene on Biological Function of Gastric Cancer Cell Line SGC7901.
机构信息
Department of Gastroenterology, Shandong Provincial Western Hospital, Jinan City, Shandong Province, China.
Department of Gastroenterology, PLA 960th Hospital (Now:Yantai Affiliated Hospital of Binzhou Medical University), Yantai City, Shandong Province, China.
出版信息
Turk J Gastroenterol. 2021 Nov;32(11):923-931. doi: 10.5152/tjg.2020.19513.
BACKGROUND
The research aimed to observe the effect of gene silencing on the proliferation, migration, cell cycle, apoptosis, and other biological functions of human gastric cancer cells with RNA interference inhibiting the expression of the far upstream element-binding protein 1 (FUBP1) in the gastric cancer cell line SGC7901.
METHODS
The shRNA lentivirus vector of the target gene FUBP1 was constructed to transfect the gastric cancer cell line SGC7901. The qRT-PCR and western blot assays were used to detect the expression levels of FUBP1 mRNA and protein in the gastric cancer cells. The CCK-8 assay was used to detect the proliferation of gastric cancer cells. The cell scratch assay and the transwell assays were used to detect the migration of gastric cancer cells. Flow cytometry was used to detect cell cycle distribution and apoptosis.
RESULTS
The shRNA lentiviral vector of FUBP1 was successfully transfected into the gastric cancer cell line SGC7901, and could effectively reduce the expression of mRNA and protein of FUBP1. The silencing of FUBP1 could inhibit the gastric cancer cell proliferation and affect the distribution of the cell cycle, resulting in S-phase arrest and cell growth inhibition. However, FUBP1 silencing has no significant effect on cell apoptosis and migration.
CONCLUSIONS
The expression of FUBP1 can be inhibited specifically and effectively by RNA interference technology, which can significantly affect the biological function of the gastric cancer cell line SGC7901.
背景
本研究旨在观察 RNA 干扰抑制胃癌细胞系 SGC7901 中远上游元件结合蛋白 1（FUBP1）表达后，对人胃癌细胞增殖、迁移、细胞周期、凋亡等生物学功能的影响。
方法
构建靶向基因 FUBP1 的 shRNA 慢病毒载体，转染胃癌细胞系 SGC7901。qRT-PCR 和 Western blot 检测胃癌细胞中 FUBP1 mRNA 和蛋白的表达水平。CCK-8 检测胃癌细胞的增殖情况。细胞划痕实验和 Transwell 检测胃癌细胞的迁移能力。流式细胞术检测细胞周期分布和凋亡。
结果
FUBP1 的 shRNA 慢病毒载体成功转染胃癌细胞系 SGC7901，能有效降低 FUBP1 mRNA 和蛋白的表达。FUBP1 的沉默能抑制胃癌细胞的增殖，并影响细胞周期的分布，导致 S 期阻滞和细胞生长抑制。然而，FUBP1 沉默对细胞凋亡和迁移没有明显影响。
结论
RNA 干扰技术能特异性、有效地抑制 FUBP1 的表达，显著影响胃癌细胞系 SGC7901 的生物学功能。
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