人类血红蛋白的T细胞识别。通过综合合成策略定位β链的完整T细胞识别谱。
T-cell recognition of human haemoglobin. Localization of the full T-cell recognition profile of the beta-chain by a comprehensive synthetic strategy.
作者信息
Yoshioka M, Yoshioka N, Atassi M Z
出版信息
Biochem J. 1986 Mar 1;234(2):449-52. doi: 10.1042/bj2340449.
Abstract
This paper reports the localization of the regions on the beta-chain that are recognized by T cells from mice immunized with haemoglobin. The 14 overlapping peptides encompassing the entire beta-chain were examined in vitro for their ability to stimulate lymph-node cells from haemoglobin-primed B10.D2 (H-2d) and SJL (H-2s) mice. Several regions of the molecule (T sites) were found to stimulate haemoglobin-primed lymph-node cells. This strategy has enabled the localization of the full profile of T-cell recognition of the beta-chain by these mouse strains. Some of the regions that stimulated T cells appeared to coincide with those recognized by antibodies (i.e. B cells). It is noteworthy that, in addition to sites recognized by both T and B cells, the protein has other sites that are recognized exclusively by T cells and to which no detectable antibody response is directed.
摘要
本文报道了用血红蛋白免疫的小鼠T细胞所识别的β链上区域的定位。体外检测了涵盖整个β链的14个重叠肽刺激来自用血红蛋白致敏的B10.D2（H-2d）和SJL（H-2s）小鼠的淋巴结细胞的能力。发现该分子的几个区域（T位点）可刺激用血红蛋白致敏的淋巴结细胞。此策略已实现对这些小鼠品系β链T细胞识别全貌的定位。一些刺激T细胞的区域似乎与抗体（即B细胞）所识别的区域一致。值得注意的是，除了T细胞和B细胞都识别的位点外，该蛋白质还有仅被T细胞识别且未检测到抗体反应的其他位点。
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本文引用的文献
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