Sivilotti L
Brain Res Bull. 1986 Apr;16(4):553-6. doi: 10.1016/0361-9230(86)90188-7.
A new method for maintaining the isolated frog optic tectum superfused in vitro at a low temperature is described. The preparation remains viable for several hours with electrophysiological responses similar to those of the in vivo tectum. This superfusion technique will allow controlled application of putative neurotransmitters and other substances in known concentrations and therefore makes the in vitro frog optic tectum a useful model system for neuropharmacological investigations into central optic pathways.
本文描述了一种在低温下体外灌流离体青蛙视顶盖的新方法。该标本在数小时内保持存活，其电生理反应与体内视顶盖相似。这种灌流技术能够以已知浓度可控地应用假定的神经递质和其他物质，因此使体外青蛙视顶盖成为研究中枢视觉通路神经药理学的有用模型系统。
