Wang Menglun, Zhu Bin, Zhang Lei, Xiao Yutao, Liang Pei, Wu Kongming
Department of Entomology, China Agricultural University, Beijing, 100193, China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.
Insect Sci. 2022 Apr;29(2):496-504. doi: 10.1111/1744-7917.12987. Epub 2022 Jan 3.
The diamondback moth, Plutella xylostella (L.), is one of the most destructive migratory pest species of cruciferous vegetables worldwide and has developed resistance to most of the insecticides used for its control. The migration regularity, migratory behavior, and relationship between flight and reproduction of P. xylostella have been widely reported. However, the effect of migration on insecticide resistance in this pest is still unclear. In this study, the effect of migration on P. xylostella resistance to seven insecticides was investigated using populations across the Bohai Sea that were collected in the early and late seasons during 2017-2019. The bioassay results showed that the early season populations of P. xylostella from south China possessed much higher resistance to insecticides because of intensive insecticide application; alternatively, the late season populations migrated from northeast China, where the insecticides were only used occasionally, showed much lower insecticide resistance. The genome re-sequencing results revealed that, among the eight mutations involved in insecticide resistance, the frequencies of two acetylcholinesterase mutations (A298S and G324A) responsible for organophosphorus insecticide resistance were significantly decreased in the late season populations. The results indicated that P. xylostella migration between tropical and temperate regions significantly delayed the development of insecticide resistance. These findings illustrated the effect of regional migration on the evolution of insecticide resistance in P. xylostella, and provided foundational information for further research on the relationship between migration and insecticide resistance development in other insects.
小菜蛾，即小菜蛾（鳞翅目：菜蛾科），是世界范围内十字花科蔬菜最具毁灭性的迁飞性害虫之一，并且已对大多数用于防治它的杀虫剂产生了抗性。小菜蛾的迁飞规律、迁飞行为以及飞行与繁殖之间的关系已有广泛报道。然而，迁飞对这种害虫抗药性的影响仍不清楚。在本研究中，利用2017 - 2019年早季和晚季采集的渤海两岸的小菜蛾种群，研究了迁飞对小菜蛾对七种杀虫剂抗性的影响。生物测定结果表明，由于频繁使用杀虫剂，中国南方早季的小菜蛾种群对杀虫剂具有更高的抗性；相反，从中国东北迁飞而来的晚季种群，那里杀虫剂只是偶尔使用，其对杀虫剂的抗性要低得多。基因组重测序结果显示，在与抗药性相关的八个突变中，晚季种群中导致对有机磷杀虫剂产生抗性的两个乙酰胆碱酯酶突变（A298S和G324A）的频率显著降低。结果表明，小菜蛾在热带和温带地区之间的迁飞显著延缓了抗药性的发展。这些发现阐明了区域迁飞对小菜蛾抗药性进化的影响，并为进一步研究其他昆虫迁飞与抗药性发展之间的关系提供了基础信息。
