School of Public Health and Management, Ningxia Medical University, Yinchuan, 750004, China.
Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan, 750004, China.
Chin J Integr Med. 2022 Nov;28(11):975-982. doi: 10.1007/s11655-021-3309-6. Epub 2021 Dec 7.
To explore the protective effect and underlying mechanism of Lycium barbarum polysaccharides (LBP) in a non-alcoholic fatty liver disease (NAFLD) cell model.
Normal human hepatocyte LO2 cells were treated with 1 mmol/L free fatty acids (FFA) mixture for 24 h to induce NAFLD cell model. Cells were divided into 5 groups, including control, model, low-, medium- and high dose LBP (30,100 and 300 µg/mL) groups. The monosaccharide components of LBP were analyzed with high performance liquid chromatography. Effects of LBP on cell viability and intracellular lipid accumulation were assessed by cell counting Kit-8 assay and oil red O staining, respectively. Triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), adenosine triphosphate (ATP) and oxidative stress indicators were evaluated. Energy balance and mitochondrial biogenesis related mRNA and proteins were determined by quantitative real-time polymerase chain reaction and Western blot, respectively.
Heteropolysaccharides with mannose and glucose are the main components of LBP. LBP treatment significantly decreased intracellular lipid accumulation as well as TG, ALT, AST and malondialdehyde levels (P<0.05 or P<0.01), increased the levels of superoxide dismutase, phospholipid hydroperoxide glutathione peroxidase, catalase, and ATP in NAFLD cell model (P<0.05). Meanwhile, the expression of uncoupling protein 2 was down-regulated and peroxisome proliferator-activated receptor gamma coactivator-1α/nuclear respiratory factor 1/mitochondrial transcription factor A pathway was up-regulated (P<0.05).
LBP promotes mitochondrial biogenesis and improves energy balance in NAFLD cell model.
探讨枸杞多糖（LBP）在非酒精性脂肪性肝病（NAFLD）细胞模型中的保护作用及其机制。
采用 1mmol/L 游离脂肪酸（FFA）混合物处理正常人肝细胞 LO2 细胞 24 h 构建 NAFLD 细胞模型。将细胞分为 5 组，分别为对照组、模型组、低、中、高剂量 LBP（30、100 和 300μg/ml）组。采用高效液相色谱法分析 LBP 的单糖组成。通过细胞计数试剂盒-8 法和油红 O 染色分别检测 LBP 对细胞活力和细胞内脂质积聚的影响。检测甘油三酯（TG）、丙氨酸转氨酶（ALT）、天门冬氨酸转氨酶（AST）、三磷酸腺苷（ATP）和氧化应激指标。采用实时定量聚合酶链反应和 Western blot 分别测定能量平衡和线粒体生物发生相关的 mRNA 和蛋白。
LBP 主要由甘露糖和葡萄糖组成的杂多糖。LBP 处理可显著减少细胞内脂质积聚以及 TG、ALT、AST 和丙二醛水平（P<0.05 或 P<0.01），增加 NAFLD 细胞模型中超氧化物歧化酶、磷脂氢过氧化物谷胱甘肽过氧化物酶、过氧化氢酶和 ATP 的水平（P<0.05）。同时，下调解偶联蛋白 2 的表达，上调过氧化物酶体增殖物激活受体γ共激活因子-1α/核呼吸因子 1/线粒体转录因子 A 通路（P<0.05）。
LBP 可促进线粒体生物发生，改善 NAFLD 细胞模型中的能量平衡。
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