Nishimura T, Uchiyama Y, Yagi H, Hashimoto Y
J Immunol Methods. 1986 Jul 11;91(1):21-7. doi: 10.1016/0022-1759(86)90097-9.
When recombinant human interleukin 2 (r-IL-2) was given to mice by single subcutaneous (s.c.) injection it rapidly disappeared from the blood. However, administration of slowly released r-IL-2 using mini-osmotic pumps caused a significant prolongation of serum levels of IL-2. Using a method for assaying IL-2 in vivo, it was also demonstrated that both the viability and the cytotoxicity of lymphokine-activated killer (LAK) cells could be maintained at a high level in vivo by administration of slowly released r-IL-2 rather than by a single injection of r-IL-2. In addition, we successfully treated EL4-bearing mice by combination therapy consisting of LAK cells and slowly released r-IL-2.
当通过单次皮下注射将重组人白细胞介素2（r-IL-2）给予小鼠时，它会迅速从血液中消失。然而，使用微型渗透泵给予缓释r-IL-2会导致IL-2血清水平显著延长。使用一种体内检测IL-2的方法还表明，通过给予缓释r-IL-2而非单次注射r-IL-2，淋巴因子激活的杀伤（LAK）细胞的活力和细胞毒性在体内均可维持在高水平。此外，我们通过由LAK细胞和缓释r-IL-2组成的联合疗法成功治疗了荷EL4小鼠。
