Department of Cardio-vascular Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Department of Cardiac and Aorta Surgery, 159411Chengdu Third People's Hospital, Chengdu, China.
Vascular. 2023 Apr;31(2):250-256. doi: 10.1177/17085381211060172. Epub 2021 Dec 7.
This study aims to investigate the methods for rat spinal cord ischemia injury models with a high long-term survival rate.
The rats were divided into three groups: the treatment group, the control group, and the sham operation group. The treatment group had a blocked thoracic aorta (landing zone 3 by Ishimaru - T11) + aortic bypass circulation for 20 min. In the control group, the thoracic aorta at the landing zone 3 was blocked for 20 min. In the sham operation group, only thoracotomy without thoracic aortic occlusion was performed. The mean arterial blood pressure (MABP) of the thoracic aorta and caudal artery before and after thoracic aortic occlusion was monitored intraoperatively. Spinal cord function was monitored by a transcranial motor evoked potential (Tc-MEP) during the operation. Spinal cord function was evaluated by the BBB scale (Basso, Beattie, & Bresnahan locomotor rating scale) scores at multiple postoperative time points. The spinal cord sections of the rats were observed for 7 days after surgery, and the survival curves were analyzed for 28 days after surgery.
After aortic occlusion, the MABP of thoracic aorta decreased to 6% of that before occlusion, and the MABP of caudal artery decreased to 63% of that before occlusion in the treatment group. In the control group, the MABP of both thoracic aorta and caudal artery decreased to 19% of that before occlusion. The Tc-MEP waveform of the treatment group disappeared after 6 min, and that of the control group disappeared after 8 min until the end of surgery. There was no change in the Tc-MEP waveform in the sham operation group. The BBB score of the treatment group decreased more obviously than the control group, and there was a significant difference. There was no decrease in the sham group. Spinal cord sections showed a large number of degeneration and necrosis of neurons, infiltration of inflammatory cells, and proliferation of surrounding glial cells in the treatment group. In the control group, multiple neurons were necrotic. The histology of the sham operation group was normal. The 28-day survival rate of the treatment group was 73.3%, which was higher than the control group (40.0%), and there was a significant difference ( < 0.05).
Thoracic aortic occlusion combined with aortic bypass is an effective modeling method for rats with accurate modeling effects and high long-term survival rates.
本研究旨在探索一种具有高长期存活率的大鼠脊髓缺血损伤模型的制作方法。
将大鼠分为三组：治疗组、对照组和假手术组。治疗组行阻断胸主动脉（Ishimaru-T11 着陆区 3）+主动脉旁路循环 20 分钟。对照组行胸主动脉着陆区 3 阻断 20 分钟。假手术组仅行开胸手术但不阻断胸主动脉。术中监测胸主动脉和尾动脉的平均动脉血压（MABP）在胸主动脉阻断前后的变化。术中通过经颅运动诱发电位（Tc-MEP）监测脊髓功能。术后多个时间点采用 BBB 评分（Basso、Beattie 和 Bresnahan 运动评分量表）评估脊髓功能。术后第 7 天行大鼠脊髓节段观察，术后第 28 天行生存曲线分析。
主动脉阻断后，治疗组胸主动脉 MABP 下降至阻断前的 6%，尾动脉 MABP 下降至阻断前的 63%。对照组胸主动脉和尾动脉 MABP 均下降至阻断前的 19%。治疗组 Tc-MEP 波形于 6 分钟后消失，对照组于 8 分钟后消失直至手术结束。假手术组 Tc-MEP 波形无变化。治疗组 BBB 评分较对照组下降更明显，差异有统计学意义。假手术组无下降。治疗组脊髓切片显示大量神经元变性坏死，炎性细胞浸润，周围胶质细胞增生。对照组多灶性神经元坏死。假手术组组织学正常。治疗组 28 天生存率为 73.3%，高于对照组（40.0%），差异有统计学意义（<0.05）。
胸主动脉阻断联合主动脉旁路是一种有效的大鼠脊髓缺血损伤模型制作方法，具有准确的建模效果和较高的长期存活率。
