Escribano Begoña M, Muñoz-Jurado Ana, Luque Evelio, Conde Cristina, Feijóo Montse, LaTorre Manuel, Valdelvira Manuel E, Buendía Paula, Giraldo Ana I, Caballero-Villarraso Javier, Santamaría Abel, Agüera Eduardo, Túnez Isaac
Department of Cell Biology, Physiology and Immunology, Faculty of Veterinary Medicine, University of Cordoba, Cordoba, Spain.
Maimonides Institute for Research in Biomedicine of Cordoba, (IMIBC), Cordoba, Spain.
CNS Neurol Disord Drug Targets. 2022;21(8):680-692. doi: 10.2174/1871527320666211207101113.
Experimental Autoimmune Encephalomyelitis (EAE) in rats closely reproduces Multiple Sclerosis (MS), a disease characterized by neuroinflammation and oxidative stress that also appears to extend to other organs and their compartments. The origin of MS is a matter for discussion, but it would seem that altering certain bacterial populations present in the gut may lead to a proinflammatory condition due to the bacterial Lipopolysaccharides (LPS) in the so-called brain-gut axis. The casein and lactose in milk confer anti-inflammatory properties and immunomodulatory effects. The objectives of this study were to evaluate the effects of administration of casein and lactose on the oxidative damage and the clinical status caused by EAE and to verify whether both casein and lactose had any effect on the LPS and its transport protein -LBP-.
Twenty male Dark Agouti rats were divided into control rats (control), EAE rats, and EAE rats, to which casein and lactose, EAE+casein, and EAE+lactose, respectively, were administered. Fifty-one days after casein and lactose administration, the rats were sacrificed, and different organs were studied (brain, spinal cord, blood, heart, liver, kidney, small, and large intestine). In the latter, products derived from oxidative stress were studied (lipid peroxides and carbonylated proteins) as well as the glutathione redox system, various inflammation factors (total nitrite, Nuclear Factor-kappa B p65, the Rat Tumour Necrosis Factor-α), and the LPS and LBP values.
Casein and lactose administration improved the clinical aspect of the disease at the same time as reducing inflammation and oxidative stress, exerting its action on the glutathione redox system, or increasing GPx levels.
大鼠实验性自身免疫性脑脊髓炎（EAE）与多发性硬化症（MS）极为相似，MS是一种以神经炎症和氧化应激为特征的疾病，且似乎还会累及其他器官及其腔室。MS的病因尚在讨论中，但似乎由于所谓脑-肠轴中的细菌脂多糖（LPS），改变肠道中某些细菌种群可能导致促炎状态。牛奶中的酪蛋白和乳糖具有抗炎特性和免疫调节作用。本研究的目的是评估给予酪蛋白和乳糖对EAE所致氧化损伤和临床状态的影响，并验证酪蛋白和乳糖是否对LPS及其转运蛋白-LBP-有任何作用。
将20只雄性黑褐大鼠分为对照组（control）、EAE大鼠组，以及分别给予酪蛋白和乳糖的EAE大鼠组，即EAE+酪蛋白组和EAE+乳糖组。给予酪蛋白和乳糖51天后，处死大鼠，研究不同器官（脑、脊髓、血液、心脏、肝脏、肾脏、小肠和大肠）。在后者中，研究氧化应激产物（脂质过氧化物和羰基化蛋白）以及谷胱甘肽氧化还原系统、各种炎症因子（总亚硝酸盐、核因子-κB p65、大鼠肿瘤坏死因子-α），以及LPS和LBP值。
给予酪蛋白和乳糖可改善疾病的临床症状，同时减轻炎症和氧化应激，对谷胱甘肽氧化还原系统发挥作用，或提高谷胱甘肽过氧化物酶（GPx）水平。
