Departmento de Biologia Celular, Fisiologia e Inmunologia, Facultad de Veterinaria, Universidad de Cordoba, Cordoba, Spain; Instituto Maimonides de Investigacion Biomedica de Cordoba (IMIBIC), Cordoba, Spain.
Instituto Maimonides de Investigacion Biomedica de Cordoba (IMIBIC), Cordoba, Spain; Departmento de Ciencias Morfologicas, Seccion de Histologia, Facultad de Medicina y Enfermeria, Universidad de Cordoba, Cordoba, Spain.
Eur J Pharmacol. 2017 Nov 15;815:266-273. doi: 10.1016/j.ejphar.2017.09.025. Epub 2017 Sep 19.
Garlic is a component of the Mediterranean diet. S-allyl cysteine (SAC), the most common organosulphur present in garlic, possesses neuroprotective properties. This investigation was performed to evaluate the dose-dependent protective action of SAC on oxidative damage, inflammation and gut microbiota alterations biomarkers. Experimental autoimmune encephalomyelitis (EAE) as a model of multiple sclerosis (MS) was induced by the myelin oligodendrocyte glycoprotein (MOG), whose effects were quantified by examining the changes in: clinical score, lipid peroxidation products, carbonylated proteins, glutathione system, tumor necrosis factor alpha (TNFα), and lipopolysaccharide membrane bacteria (LPS). Our results reveal that MOG induces paralysis, oxidative damage and increases in LPS binding protein (LBP) and LPS levels. In this work, two doses of SAC were compared with two dose of N-acetyl cysteine (NAC). SAC was more effective than NAC and it prevented the harmful effects induced by MOG more effectively at the dose of 50mg/kg than that of 18mg/kg. Surprisingly, NAC increases LBP levels while SAC had not such negative effect. In conclusion the data show the ability of SAC to modify EAE evolution.
大蒜是地中海饮食的组成部分。S-烯丙基半胱氨酸（SAC）是大蒜中最常见的有机硫化合物，具有神经保护作用。本研究旨在评估 SAC 对氧化损伤、炎症和肠道微生物群改变生物标志物的剂量依赖性保护作用。实验性自身免疫性脑脊髓炎（EAE）作为多发性硬化症（MS）的模型，通过髓鞘少突胶质细胞糖蛋白（MOG）诱导，通过检查以下变化来量化其作用：临床评分、脂质过氧化产物、羰基化蛋白、谷胱甘肽系统、肿瘤坏死因子α（TNFα）和脂多糖膜细菌（LPS）。我们的结果表明，MOG 诱导瘫痪、氧化损伤，并增加脂多糖结合蛋白（LBP）和 LPS 水平。在这项工作中，比较了 SAC 的两个剂量与 N-乙酰半胱氨酸（NAC）的两个剂量。SAC 比 NAC 更有效，在 50mg/kg 的剂量下比 18mg/kg 的剂量更有效地预防了 MOG 诱导的有害作用。令人惊讶的是，NAC 增加了 LBP 水平，而 SAC 没有这种负面影响。总之，数据显示了 SAC 改变 EAE 演变的能力。
