Clinical Pathology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
Biostatistics and Cancer Epidemiology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
Transfus Apher Sci. 2022 Apr;61(2):103327. doi: 10.1016/j.transci.2021.103327. Epub 2021 Nov 25.
Platelet transfusion therapy is widely used to prevent hemorrhage in patients with thrombocytopenia and platelet disorders. The platelet concentrate (PC) quality is affected by increased storage time, as reflected in the decreased number of platelets, morphological changes, and impaired functions. This study aimed to analyze the impact of 5 days storage on platelets count and the expression of CD63, and Annexin V as activation markers during PC storage.
Fifty PCs collected from single donors were tested for platelet count on days 0, 3, and 5 using a Sysmex blood counter. CD61, CD63, and Annexin V expression was analyzed by a multicolor Navios flow cytometer.
There was a significant decrease in platelet count during 5 days of storage. There was a direct relationship between storage time and degree of platelet activation. CD63 had almost double increased expression on day 5 than day 3. Annexin V showed significantly increased expression on day 3 with minor differences between days 3 and 5.
According to standard blood bank conditions, PC stored for 5 days showed a degree of in vitro activation as evidenced by CD63 and Annexin V expression, may lead to reduced therapeutic efficacy. Flow cytometry monitoring platelet activation in PC offers a better understanding of the changes during PC storage and may help improve platelet products.
血小板输注疗法广泛用于预防血小板减少症和血小板功能障碍患者的出血。血小板浓缩物 (PC) 的质量受到储存时间延长的影响，表现为血小板数量减少、形态变化和功能受损。本研究旨在分析 PC 储存 5 天对血小板计数以及 CD63 和 Annexin V 作为活化标志物表达的影响。
使用 Sysmex 血液计数器在第 0、3 和 5 天检测 50 份来自单个供体的 PC 中的血小板计数。通过多色 Navios 流式细胞仪分析 CD61、CD63 和 Annexin V 的表达。
在 5 天的储存过程中，血小板计数显著下降。储存时间与血小板活化程度之间存在直接关系。CD63 在第 5 天的表达几乎是第 3 天的两倍。第 3 天 Annexin V 的表达显著增加，第 3 天和第 5 天之间差异较小。
根据标准血库条件，PC 储存 5 天表现出体外活化程度，如 CD63 和 Annexin V 表达所示，可能导致治疗效果降低。流式细胞术监测 PC 中的血小板活化可更好地了解 PC 储存过程中的变化，并有助于改善血小板产品。
