Greer C N, Shewen P E
Vet Microbiol. 1986 Jun;12(1):33-42. doi: 10.1016/0378-1135(86)90039-8.
A rapid and easily performed colorimetric method has been adapted for the detection of the Pasteurella haemolytica leucotoxin using the lysis of bovine leucocyte BL-3 cells (a bovine lymphoid cell line obtained from Dr. G. Theilen, University of California, Davis). The method involves incubating BL-3 cells in the presence of P. haemolytica culture supernatant and assessing cell survival at the end of the assay by staining remaining BL-3 cells with the dye neutral red. Solubilization of the cells with concomitant dye release allows the assay to be read by measuring optical density (540 nm) with an automated spectrophotometer. This assay can also be used for the determination of neutralizing antiserum titres.
一种快速且易于操作的比色法已被用于检测溶血巴斯德氏菌白细胞毒素，该方法利用牛白细胞BL - 3细胞（一种从加利福尼亚大学戴维斯分校的G. 泰伦博士处获得的牛淋巴细胞系）的裂解。该方法包括在溶血巴斯德氏菌培养上清液存在的情况下孵育BL - 3细胞，并在测定结束时通过用染料中性红对剩余的BL - 3细胞进行染色来评估细胞存活情况。细胞溶解并伴随染料释放，使得可以通过使用自动分光光度计测量光密度（540nm）来读取测定结果。该测定法还可用于测定中和抗血清滴度。
