Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, Oyo State, Nigeria.
Department of Computational and Analytical Sciences, Rothamsted Research, Hertfordshire, United Kingdom.
Nat Prod Res. 2022 Sep;36(18):4681-4691. doi: 10.1080/14786419.2021.2012774. Epub 2021 Dec 8.
species are known edible mushrooms in Nigeria, believed to have exceptional culinary and nutraceutical properties. Methanol extract from fruiting bodies of was evaluated for antidiabetic activity using α-amylase and α-glucosidase assays. The isolation and structural elucidation of metabolites from the . extract afforded five compounds including a new natural product γ-glutamyl-β-phenylethylamine and four known phenyl derivatives: tryptophan , 4-hydroxyphenylacetic acid , 4-hydroxyphenylpropionic acid , and phenyllactic acid . Structures were elucidated from analyses of spectroscopic data (1D and 2D NMR, HRESIMS) and all isolated compounds were tested for α-amylase and α-glycosidase inhibitory activity. The assay established crude extract to possess and inhibition with IC of 78.05µg/mL and 86.10µg/mL, respectively. The isolated compounds compared favourably with the standard drug, acarbose with IC ranging from 6.18-15.08µg/mL and 18.28-44.63µg/mL for α-amylase and glucosidase, respectively.
在尼日利亚，已知有一些物种是可食用的蘑菇，被认为具有特殊的烹饪和营养特性。使用 α-淀粉酶和 α-葡萄糖苷酶测定法评估了来自子实体的甲醇提取物的抗糖尿病活性。从. 提取物中分离和结构鉴定代谢产物，得到了五个化合物，包括一个新的天然产物 γ-谷氨酰-β-苯乙胺和四个已知的苯衍生物：色氨酸、4-羟基苯乙酸、4-羟基苯丙酸和苯乳酸。结构通过光谱数据分析（1D 和 2D NMR、HRESIMS）阐明，所有分离的化合物均测试了对 α-淀粉酶和 α-葡萄糖苷酶的抑制活性。 测定法确定粗提取物分别具有 和 抑制作用，IC 分别为 78.05µg/mL 和 86.10µg/mL。与标准药物阿卡波糖相比，分离得到的化合物的 IC 范围分别为 6.18-15.08µg/mL 和 18.28-44.63µg/mL，用于α-淀粉酶和葡萄糖苷酶。
