Cheng Zehua, Gu Liqiang, Zhao Yunyang, Yang Lele, Chen Ling, Wang Ting, Luo Mai, Wei Jinchao, Li Peng
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China.
Joint Key Laboratory of the Ministry of Education, Institute of Applied Physics and Materials Engineering, University of Macau, Macau, China.
J Hazard Mater. 2022 Feb 15;424(Pt B):127555. doi: 10.1016/j.jhazmat.2021.127555. Epub 2021 Oct 20.
Pesticide residue contamination has become an urgent issue since it threatens both the natural environment and public health. In this study, a fluorescent method for detecting dithiocarbamate (DTC) compounds was constructed based on novel nickel nanoclusters (Ni NCs) and copper ions (Cu). The water-soluble fluorescent Ni NCs were synthesized for the first time through a one-pot method using glutathione as stabilizer and ascorbic acid as reducing agent. The as-prepared Ni NCs exhibited a maximum fluorescence emission at 445 nm when excited by 380 nm. And they displayed aggregation-induced emission enhancement when ethylene glycol was introduced into the nanocluster aqueous solution. Based on the Ni NCs, a label-free fluorescence quenching sensor was established for sensitive and selective detection of DTC compounds with the assistance of Cu. The complex formed by DTC and Cu led to fluorescence quenching of Ni NCs through inner filter effect. The sensing method was successfully applied to two typical DTC compounds, thiram and disulfiram, with good linearity over a wide linear range and a low detection limit. Moreover, the proposed approach was capable of thiram detection in real samples, which confirms the potential of this sensing method as a platform for DTC compound detection.
农药残留污染已成为一个紧迫问题，因为它对自然环境和公众健康都构成威胁。在本研究中，基于新型镍纳米簇（Ni NCs）和铜离子（Cu）构建了一种检测二硫代氨基甲酸盐（DTC）化合物的荧光方法。首次以谷胱甘肽为稳定剂、抗坏血酸为还原剂，通过一锅法合成了水溶性荧光Ni NCs。制备的Ni NCs在380 nm激发下，在445 nm处呈现最大荧光发射。当将乙二醇引入纳米簇水溶液中时，它们表现出聚集诱导发射增强。基于Ni NCs，在Cu的辅助下建立了一种无标记荧光猝灭传感器，用于灵敏且选择性地检测DTC化合物。DTC与Cu形成的络合物通过内滤效应导致Ni NCs的荧光猝灭。该传感方法成功应用于两种典型的DTC化合物福美双和戒酒硫，在较宽的线性范围内具有良好的线性关系和较低的检测限。此外，所提出的方法能够检测实际样品中的福美双，这证实了该传感方法作为DTC化合物检测平台的潜力。
