Yessinou Roland Eric, Adoligbe Camus, Atchade Fréjus, Adinci Justin, Brahi Hermann Dave, Adehan Safiou, Mantip Samuel Elias, Farougou Souaïbou
University of Abomey-Calavi (UAC), Communicable Diseases Research Unit (URMAT), 01 PO Box: 2009, Cotonou, Benin.
University of Abomey-Calavi (UAC), Communicable Diseases Research Unit (URMAT), 01 PO Box: 2009, Cotonou, Benin.
Vet Parasitol Reg Stud Reports. 2021 Dec;26:100638. doi: 10.1016/j.vprsr.2021.100638. Epub 2021 Sep 11.
The aim of this study was to evaluate the phenotypic and genotypic resistance of Rhipicephalus sanguineus sensu lato to deltamethrin in dogs in the municipality of Natitingou in Benin. In this study, the collection and identification of ticks, Larval Packet Test and specific allele PCR were the main methods used. A total of 41 dogs were examined and 420 ticks were collected in six areas which includes: Tchrimina, Yokossi, Boriyoure, Berécingou, Péporiyakou and Perma. Three species of ticks were identified and they include: R. sanguineus s.l., Rhipicephalus microplus and Haemaphysalis spp. However, R. sanguineus s.l. was the most abundant tick species (9.85) and had the highest parasitic intensity (10.36). The results of the Larval Packet Test showed that the larvae of R. sanguineus s.l. developed a phenotypic resistance against deltamethrin, particularly in the Bérécingou area where resistance ratios of LC50 and LC95 of R. sanguineus s.l. compared to a susceptible strain were 1.591 (1.025-3.054) and 65.339 (20.235-579.825) respectively. The molecular study of the sodium channel gene revealed three genotypes namely the susceptible genotype (SS), the heterozygous genotype (SR) and the resistant genotype (RR), which correlates with the phenotypic results. This information is fundamental to improve monitoring and resistance management strategies of R. sanguineus s.l. ticks to pyrethroids.
本研究旨在评估贝宁纳蒂廷古市犬身上的血红扇头蜱（Rhipicephalus sanguineus sensu lato）对溴氰菊酯的表型和基因型抗性。在本研究中，蜱的采集与鉴定、幼虫包囊试验和特异性等位基因PCR是主要使用的方法。在包括奇里米纳、约科西、博里约雷、贝雷辛古、佩波里亚库和佩尔马在内的六个地区共检查了41只犬，收集到420只蜱。鉴定出三种蜱，分别为：血红扇头蜱指名亚种、微小扇头蜱和血蜱属。然而，血红扇头蜱指名亚种是最常见的蜱种（9.85），且寄生强度最高（10.36）。幼虫包囊试验结果表明，血红扇头蜱指名亚种的幼虫对溴氰菊酯产生了表型抗性，尤其是在贝雷辛古地区，与敏感品系相比，血红扇头蜱指名亚种的LC50和LC95抗性比率分别为1.591（1.025 - 3.054）和65.339（20.235 - 579.825）。钠通道基因的分子研究揭示了三种基因型，即敏感基因型（SS）、杂合基因型（SR）和抗性基因型（RR），这与表型结果相关。这些信息对于改进血红扇头蜱指名亚种对拟除虫菊酯的监测和抗性管理策略至关重要。
