伴有和不伴有血小板增多症的癌性犬的白细胞介素-6 和血小板生成素浓度。

Interleukin-6 and thrombopoietin concentrations in dogs with carcinoma with and without thrombocytosis.

机构信息

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA.

Department of Veterinary Administration, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA.

出版信息

J Vet Intern Med. 2022 Jan;36(1):227-233. doi: 10.1111/jvim.16317. Epub 2021 Dec 8.

DOI:10.1111/jvim.16317

PMID:34881459

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8783347/

Abstract

BACKGROUND

Carcinoma-associated thrombocytosis involves tumor production of mediators such as interleukin-6 (IL-6) and thrombopoietin (TPO) that increase thrombopoiesis and may play a role in tumor evasion and metastasis. Carcinoma-associated thrombocytosis is described in people, but has not been described in dogs.

HYPOTHESIS/OBJECTIVES: Evaluate the concentrations of IL-6 and TPO in dogs diagnosed with carcinoma with or without thrombocytosis. We hypothesized that IL-6 and TPO concentrations would be higher in dogs with carcinoma compared to healthy dogs, and that IL-6 and TPO concentrations would be higher in dogs with carcinoma and thrombocytosis when compared to dogs with carcinoma and normal platelet counts.

ANIMALS

One-hundred sixteen dogs: 63 with carcinoma and 53 healthy control dogs.

METHODS

Complete blood count was performed in all dogs, and they were stratified for sub-group analysis based on the presence or absence of thrombocytosis (platelet count > 500 103/µL). Serum TPO and IL-6 concentrations were measured by ELISA. Results of selected numeric variables were compared using Wilcoxon rank sum tests for pairwise comparisons. A value of P < .05 was considered significant.

RESULTS

Twelve of the dogs with carcinoma (12/63, 19.0%) and none of the healthy control dogs (0%) had thrombocytosis. Thrombopoietin concentrations (median [range]) were significantly higher in dogs with carcinoma when compared to controls (87.42 pg/mL [0 to >600] vs 15.99 pg/mL [0 to >600], P < .001). Interleukin-6 concentrations (median [range]) were not different between dogs with carcinoma and healthy control dogs (9.70 pg/mL [0-181.53] vs 3.03 pg/mL [0-280.77], P = .15). In dogs with carcinoma, the TPO and IL-6 concentrations were not different between dogs with thrombocytosis and dogs with normal platelet count.

CONCLUSIONS AND CLINICAL IMPORTANCE

Thrombopoietin concentrations were significantly higher in dogs with carcinoma, regardless of platelet count. Thrombopoietin is likely to be 1 of multiple factors that can impact platelet number, production, and consumption in dogs with carcinoma.

摘要

背景

癌相关性血小板增多症涉及肿瘤产生的介质，如白细胞介素-6（IL-6）和血小板生成素（TPO），这些介质可增加血小板生成，并可能在肿瘤逃逸和转移中发挥作用。癌相关性血小板增多症在人类中已有描述，但在犬中尚未描述。

假设/目的：评估诊断为癌伴或不伴血小板增多症的犬的 IL-6 和 TPO 浓度。我们假设癌相关血小板增多症的犬的 IL-6 和 TPO 浓度会高于健康犬，并且癌相关血小板增多症的犬的 IL-6 和 TPO 浓度会高于癌伴正常血小板计数的犬。

动物

116 只犬：63 只患有癌，53 只健康对照犬。

方法

对所有犬进行全血细胞计数，并根据是否存在血小板增多症（血小板计数>500×103/μL）进行亚组分析。通过 ELISA 测量血清 TPO 和 IL-6 浓度。使用 Wilcoxon 秩和检验对选定的数值变量进行比较，双侧 P 值<0.05 被认为有统计学意义。

结果

12 只癌相关犬（12/63，19.0%）和 0 只健康对照犬（0%）有血小板增多症。与对照组相比，患有癌的犬的 TPO 浓度（中位数[范围]）明显更高（87.42pg/mL[0 至>600]比 15.99pg/mL[0 至>600]，P<0.001）。与健康对照犬相比，患有癌的犬的 IL-6 浓度（中位数[范围]）没有差异（9.70pg/mL[0-181.53]比 3.03pg/mL[0-280.77]，P=0.15）。在患有癌的犬中，血小板增多症与正常血小板计数的犬的 TPO 和 IL-6 浓度没有差异。