Clinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich, Switzerland; Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Egypt.
Clinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich, Switzerland.
Reprod Biol. 2022 Jun;22(2):100577. doi: 10.1016/j.repbio.2021.100577. Epub 2021 Dec 6.
This study aimed to investigate the susceptibility to persistent breeding-induced endometritis (PBIE). Cytobrush samples were collected from 81 broodmares 1-3 days before artificial insemination (AI). Susceptibility to PBIE was evaluated by the presence of ≥ 2 cm of intrauterine fluid 24 h after AI, besides the fertility was determined by a sonographic pregnancy diagnosis 2 weeks after ovulation. RNA expressions were compared between susceptible non-pregnant (SNP) mares (n=9) and resistant pregnant (RP) mares (n=9) as well as between susceptible pregnant (SP) mares (n=9) and susceptible non-pregnant (SNP) mares. 66 differentially expressed genes (DEGs) were identified between SNP and RP mares and 60 DEGs between SP and SNP mares. In SNP compared to RP mares, transcript levels of genes regulating steroid hormone metabolism and neutrophil chemotaxis were lower, while higher for genes participating in uterine inflammation.Transcripts of genes related to extracellular matrix degradation, tissue adhesions, and fibrosis were lower in SP mares than in SNP mares, while higher for genes related to uterine cell proliferation, differentiation, and angiogenesis in SP mares than SNP mares. In conclusion, increased transcript levels of apolipoprotein E (APOE) and roundabout 2 (ROBO2), cluster domain 44 (CD44), integrin beta 3 (ITGB3), and epidermal growth factor (EGF) are possible biomarkers for susceptibility to PBIE. While higher expression of fibroblast growth factor 9 (FGF9), kinase domain receptor (KDR), and C-X-C motif chemokine ligand (CXCL) 16, collagen type V alpha 2 (COL5A2) and fibronectin (FN1) are suggested indicators of fertility in susceptible mares if they receive proper breeding management.
本研究旨在探究持续性繁殖诱导的子宫内膜炎（PBIE）易感性。在人工授精（AI）前 1-3 天，从 81 匹母马中采集细胞刷样本。通过 AI 后 24 小时宫内有≥2cm 的液体来评估 PBIE 的易感性，同时通过排卵后 2 周的超声妊娠诊断来确定繁殖力。比较了易感未妊娠（SNP）母马（n=9）和抗孕妊娠（RP）母马（n=9）以及易感妊娠（SP）母马（n=9）和易感未妊娠（SNP）母马之间的 RNA 表达。SNP 和 RP 母马之间鉴定出 66 个差异表达基因（DEG），SP 和 SNP 母马之间鉴定出 60 个 DEG。与 RP 母马相比，SNP 母马中调节类固醇激素代谢和中性粒细胞趋化的基因转录水平较低，而参与子宫炎症的基因转录水平较高。与 SNP 母马相比，SP 母马中外泌体基质降解、组织粘连和纤维化相关基因的转录水平较低，而 SP 母马中与子宫细胞增殖、分化和血管生成相关的基因转录水平较高。总之，载脂蛋白 E（APOE）和绕路蛋白 2（ROBO2）、簇域 44（CD44）、整合素β 3（ITGB3）和表皮生长因子（EGF）的转录水平增加可能是 PBIE 易感性的生物标志物。而如果接受适当的繁殖管理，较高的成纤维细胞生长因子 9（FGF9）、激酶结构域受体（KDR）和 C-X-C 基序趋化因子配体（CXCL）16、胶原 V 型α 2（COL5A2）和纤维连接蛋白（FN1）的表达可能是易感母马繁殖力的指标。
