DIMEVET, Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano dell'Emilia, BO, Italy.
AUB INFA, National Institute of Artificial Insemination, University of Bologna, Via Gandolfi 16, 40057, Granarolo dell'Emilia, BO, Italy.
Theriogenology. 2022 Feb;179:204-210. doi: 10.1016/j.theriogenology.2021.12.001. Epub 2021 Dec 2.
Many mares are susceptible to persistent mating-induced endometritis (PMIE), an important cause of reduced fertility. Platelet lysate (PL) derives from freeze-thawing platelets after concentration, so that growth factors are released from the platelets. Among the advantages of PL compared to platelet-rich plasma (PRP), it can be frozen stored and allogenic use for PL might also be conceivable. Platelet-rich plasma beneficially reduced inflammatory response in PMIE mares when administered 24 h pre- or 4 h post-AI. The aim of this study was to test the effect of PL on inflammatory uterine response in mares susceptible to PMIE. A total of 14 mares susceptible to PMIE (based on presence of fluid or inflammatory cells 24 h after AI) underwent an untreated (Ctr) cycle followed by a treated (PL) cycle. From each mare, 100 mL of citrated whole blood was obtained for PRP production by centrifugation. The resultant PRP was brought to a final volume of 10 mL with platelet poor plasma and frozen at -80 °C to obtain PL. On untreated cycles, mares were inseminated with frozen-thawed semen 36 h after ovulation induction. On treated cycles, PL was thawed, infused into the uterus 12 h after ovulation induction, and AIs were performed 24 h later. The number of neutrophils in uterine cytology (score 1(normal)-3(severe inflammation)) evaluated by optical microscopy, uterine fluid accumulation (height x width) and uterine edema (score 0-3) observed in ultrasonography, were analysed. Pregnancy was evaluated by ultrasonography 14 days after ovulation. A significant decrease (P < 0.05) was observed on cytology score (PL 1.3 ± 0.1 vs Ctr 2.0 ± 0.1), fluid accumulation (PL 79.5 ± 30.1 mm vs Ctr 342.7 ± 52.9 mm) and edema score (PL 1.8 ± 0.2 vs Ctr 2.3 ± 0.2) in treated mares. Pregnancy rate in PL-treated cycles (3/12) and control cycles (2/14), were not significantly different (P > 0.05). According to the results, we conclude that treatment with PL in mares classified as susceptible to PMIE appears to reduce the inflammatory response after breeding, based on clinical signs of uterine edema, IUF accumulation and PMNs migration.
许多母马易患持续性配种诱导的子宫内膜炎（PMIE），这是降低生育力的一个重要原因。血小板裂解液（PL）来源于血小板经浓缩后的冻融，从而使血小板释放生长因子。与富血小板血浆（PRP）相比，PL 的优势在于可以冷冻保存，同种异体使用 PL 也可能是可行的。PRP 在 AI 前 24 小时或 AI 后 4 小时给予 PMIE 母马时，可降低其炎症反应。本研究旨在检测 PL 对易感 PMIE 的母马子宫炎症反应的影响。共有 14 匹易感 PMIE 的母马（根据 AI 后 24 小时的液体或炎症细胞存在情况进行分类）接受了未治疗（Ctr）周期和治疗（PL）周期。从每匹马中采集 100 毫升枸橼酸盐全血，通过离心获得 PRP。所得 PRP 用血小板贫血浆稀释至终体积 10ml，然后在-80°C 冷冻获得 PL。在未治疗周期中，母马在排卵诱导后 36 小时进行冻精授精。在治疗周期中，PL 解冻后在排卵诱导后 12 小时注入子宫，24 小时后进行 AI。光学显微镜评估的子宫细胞学中性粒细胞数量（评分 1（正常）-3（严重炎症））、超声观察到的子宫液体积聚（高度 x 宽度）和子宫水肿（评分 0-3）。排卵后 14 天通过超声评估妊娠情况。细胞学评分（PL 1.3±0.1 与 Ctr 2.0±0.1）、液体积聚（PL 79.5±30.1mm 与 Ctr 342.7±52.9mm）和水肿评分（PL 1.8±0.2 与 Ctr 2.3±0.2）均显著降低（P<0.05）。PL 治疗周期（3/12）和对照周期（2/14）的妊娠率无显著差异（P>0.05）。根据结果，我们得出结论，在被归类为易感 PMIE 的母马中，PL 治疗似乎可降低发情后配种的炎症反应，根据子宫水肿、IUF 积聚和 PMN 迁移的临床迹象。
