Hunan Mid-Subtropical Quality Plant Breeding and Utilization Engineering Technology Research Center, College of Horticulture, Hunan Agriculture University, Changsha 410128, China.
Institute of Agriculture Environment and Agro Ecology, Hunan Academy of Agriculture Sciences, Changsha 410125, China.
Int J Mol Sci. 2021 Nov 29;22(23):12929. doi: 10.3390/ijms222312929.
The photoperiodic flowering pathway is essential for plant reproduction. As blue and ultraviolet-A light receptors, cryptochromes play an important role in the photoperiodic regulation of flowering. is an important cut flower that flowers within a year after seed propagation. Floral induction is highly sensitive to photoperiod. In this study, we isolated the gene () from . The predicted LfCRY2 protein was highly homologous to other CRY2 proteins. The transcription of was induced by blue light. exhibits its highest diurnal expression during the floral induction stage under both long-day and short-day photoperiods. Overexpression of in promoted flowering under long days but not short days, and inhibited hypocotyl elongation under blue light. Furthermore, LfCRY2 was located in the nucleus and could interact with CONSTANS-like 9 (LfCOL9) and CRY-interacting basic-helix-loop-helix 1 (AtCIB1) in both yeast and onion cells, which supports the hypothesis that hastens the floral transition via the CIB1-CO pathway in a manner similar to . These results provide evidence that plays a vital role in promoting flowering under long days in .
光周期开花途径对于植物繁殖至关重要。作为蓝光和紫外 A 光受体，隐花色素在光周期调控开花中发挥着重要作用。是一种重要的切花，种子繁殖后一年内开花。花诱导对光周期高度敏感。本研究从 中分离出 基因（）。预测的 LfCRY2 蛋白与其他 CRY2 蛋白高度同源。转录物被蓝光诱导。在长日和短日光照条件下，在花诱导阶段的表达量最高。在长日照下过表达 可促进开花，但在短日照下不能促进开花，并且在蓝光下抑制下胚轴伸长。此外，LfCRY2 位于细胞核中，可在酵母和洋葱细胞中与 CONSTANS-like 9 (LfCOL9) 和 CRY-interacting basic-helix-loop-helix 1 (AtCIB1) 相互作用，这支持了 LfCRY2 通过 CIB1-CO 途径加速花转变的假说，类似于 。这些结果表明 在 中促进长日照下开花起着至关重要的作用。
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