Liu Zigang, Dong Xiaoyun, Zheng Guoqiang, Xu Chunmei, Wei Jiaping, Cui Junmei, Cao Xiaodong, Li Hui, Fang Xinlin, Wang Ying, Tian Haiyan
State Key Laboratory of Arid Land Crop Sciences, Gansu Agricultural University, Lanzhou, China.
Front Plant Sci. 2022 Jun 28;13:904198. doi: 10.3389/fpls.2022.904198. eCollection 2022.
Flowering at the proper time is an important part of acclimation to the ambient environment and season and maximizes the plant yield. To reveal the genetic architecture and molecular regulation of flowering time in oilseed rape (), we performed an RNA-seq analysis of the two parents after vernalization at low temperature and combined this with quantitative trait loci (QTL) mapping in an F population. A genetic linkage map that included 1,017 markers merged into 268 bins and covered 793.53 cM was constructed. Two QTLs associated with flowering time were detected in the F population. qFTA06 was the major QTL in the 7.06 Mb interval on chromosome A06 and accounted for 19.3% of the phenotypic variation. qFTC08 was located on chromosome C06 and accounted for 8.6% of the phenotypic variation. RNA-seq analysis revealed 4,626 differentially expressed genes (DEGs) between two parents during vernalization. Integration between QTL mapping and RNA-seq analysis revealed six candidate genes involved in the regulation of flowering time through the circadian clock/photoperiod, auxin and ABA hormone signal, and cold signal transduction and vernalization pathways. These results provide insights into the molecular genetic architecture of flowering time in .
在适宜时间开花是植物适应环境和季节的重要环节，且能使作物产量最大化。为揭示油菜开花时间的遗传结构和分子调控机制，我们对低温春化处理后的两个亲本进行了RNA测序分析，并结合F群体的数量性状位点（QTL）定位。构建了一个包含1017个标记、合并为268个bin、覆盖793.53 cM的遗传连锁图谱。在F群体中检测到两个与开花时间相关的QTL。qFTA06是A06染色体上7.06 Mb区间的主要QTL，占表型变异的19.3%。qFTC08位于C06染色体上，占表型变异的8.6%。RNA测序分析揭示了春化过程中两个亲本之间有4626个差异表达基因（DEG）。QTL定位与RNA测序分析相结合，揭示了6个通过生物钟/光周期、生长素和脱落酸激素信号以及冷信号转导和春化途径参与开花时间调控的候选基因。这些结果为油菜开花时间的分子遗传结构提供了见解。
