Nassar R, Wallace N R, Taylor I, Sommer J R
Scan Electron Microsc. 1986(Pt 1):309-28.
Single intact frog skeletal muscle fibers quick-frozen after known time intervals following electrical stimulation are examined electron microscopically in thin sections, after freeze-substitution, in freeze-fracture/etch preparations, and in cryosections prepared for x-ray microprobe analysis. Techniques are described to perform these operations on a single fiber. Notable morphological differences between conventionally fixed and cryopreserved muscle fibers, and between fibers quick-frozen at different post-stimulation intervals are demonstrated.
对电刺激后在已知时间间隔快速冷冻的单个完整青蛙骨骼肌纤维，在冷冻置换后，在冷冻断裂/蚀刻制剂中，以及在为X射线微探针分析制备的冷冻切片中进行薄切片电子显微镜检查。描述了在单根纤维上进行这些操作的技术。展示了传统固定和冷冻保存的肌纤维之间，以及在不同刺激后间隔快速冷冻的纤维之间显著的形态学差异。
