Xia Yu, Zhang Yanxia, Wang Huiyun
Department of Health Comprehensive Geriatrics, Yidu Central Hospital of Weifang, Weifang, Shandong, People's Republic of China.
Diabetes Metab Syndr Obes. 2021 Dec 3;14:4747-4756. doi: 10.2147/DMSO.S333431. eCollection 2021.
Non-alcoholic fatty liver disease (NAFLD) is a disease associated with genetic-environmental-metabolic stress, which severely damages the liver function of patients. This study aimed to explore the significance and probable functions of HCG18 in NAFLD.
The expression of HCG18 and miR-197-3p was tested by qRT-PCR. The clinical signification of HCG18 was provided by the ROC curve and Pearson correlation. The corresponding mechanism was punctuated by the luciferase reporter assay and HFD-managed mice.
HCG18 expression was higher in the patients with NAFLD than in controls and in individuals with HOMA-IR score ≥2.5 than those with HOMA-IR score <2.5. HCG18 expression in NAFLD patients was related to BMI, HOMA-IR, ALT, FBG, TC, and TG. HCG18 showed satisfactory predictive accuracy in differentiating NAFLD patients and patients with HOMA-IR ≥2.5. Besides, HCG18 had protective impacts on blood glucose and fat deposition but not on body weight. MiR-197-3p is a direct gene of HCG18, and a reverse correlation was found between miR-197-3p and HCG18. Furthermore, miR-197-3p regulated the influence of HCG18 on insulin resistance and lipid accumulation.
Increased levels of HCG18 might be an alternate indicator for NAFLD patients. The HCG18-miR-197-3p axis exerted effects on the progression of fat sedimentation and glucose disorder in NAFLD.
非酒精性脂肪性肝病（NAFLD）是一种与遗传-环境-代谢应激相关的疾病，严重损害患者的肝功能。本研究旨在探讨HCG18在NAFLD中的意义及可能的作用。
采用qRT-PCR检测HCG18和miR-197-3p的表达。通过ROC曲线和Pearson相关性分析提供HCG18的临床意义。通过荧光素酶报告基因检测和高脂饮食处理的小鼠来阐明相应机制。
NAFLD患者中HCG18的表达高于对照组，且稳态模型评估的胰岛素抵抗（HOMA-IR）评分≥2.5的个体中HCG18的表达高于HOMA-IR评分<2.5的个体。NAFLD患者中HCG18的表达与体重指数（BMI）、HOMA-IR、谷丙转氨酶（ALT）、空腹血糖（FBG）、总胆固醇（TC）和甘油三酯（TG）相关。HCG18在区分NAFLD患者和HOMA-IR≥2.5的患者方面显示出令人满意的预测准确性。此外，HCG18对血糖和脂肪沉积有保护作用，但对体重没有影响。miR-197-3p是HCG18的直接靶基因，且miR-197-3p与HCG18呈负相关。此外，miR-197-3p调节了HCG18对胰岛素抵抗和脂质蓄积的影响。
HCG18水平升高可能是NAFLD患者的一个替代指标。HCG18-miR-197-3p轴对NAFLD中脂肪沉积和糖代谢紊乱的进展发挥作用。
