Foodborne and Waterborne Diseases Research Centre, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Centre, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Folia Parasitol (Praha). 2021 Nov 23;68:2021.026. doi: 10.14411/fp.2021.026.
Apoptosis plays crucial role in the pathogenesis of toxoplasmosis, as it limits further development of the disease. The current study aimed to investigate the effects of different concentrations of soluble total antigen (STAg) of Toxoplasma gondii (Nicolle et Manceaux, 1908) on the apoptotic and anti-apoptotic pathways. PMA-activated THP-1 cell line was sensed by T. gondii STAg and the expression patterns of caspase-3, -7, -8, -9, Bax, Bcl-2, and Mcl-1 genes were evaluated. The results showed statistically significant concentration-dependent overexpression of both Bcl-2 (P-value < 0.0001) and Mcl-1 (P-value = 0.0147). The cas-7 showed overexpression in all concentrations (P-value < 0.0001). The cas-3 was suppressed in concentrations 100, 80, and 40 µg, but statistically significant downregulated in concentrations 10 and 20 µg. The Bax was suppressed in concentrations 100 to 20 µg, while it slightly downregulated 1.42 fold (P-value = 0.0029) in concentration 10 µg. The expression of cas-8 and -9 was suppressed in all concentrations. Our results indicated that T. gondii STAg downregulated and suppressed apoptotic and upregulated anti-apoptotic pathways. The upregulation of cas-7 in this study may indicate the role of T. gondii STAg in activation of inflammatory responses.
细胞凋亡在弓形虫病的发病机制中起着至关重要的作用，因为它限制了疾病的进一步发展。本研究旨在探讨不同浓度的弓形虫可溶性总抗原（STAg）对凋亡和抗凋亡途径的影响。用 T. gondii STAg 感应 PMA 激活的 THP-1 细胞系，并评估 caspase-3、-7、-8、-9、Bax、Bcl-2 和 Mcl-1 基因的表达模式。结果显示，Bcl-2（P 值<0.0001）和 Mcl-1（P 值=0.0147）均呈浓度依赖性过表达，且具有统计学意义。所有浓度的 cas-7 均过表达（P 值<0.0001）。caspase-3 在浓度 100、80 和 40 µg 时被抑制，但在浓度 10 和 20 µg 时被显著下调。Bax 在浓度 100 至 20 µg 时被抑制，而在浓度 10 µg 时略有下调 1.42 倍（P 值=0.0029）。caspase-8 和 -9 的表达在所有浓度下均受到抑制。我们的结果表明，T. gondii STAg 下调并抑制凋亡，上调抗凋亡途径。本研究中 cas-7 的上调可能表明 T. gondii STAg 在激活炎症反应中的作用。
