Chen L K, Bensussan A, Burns G F, Tourvieille B, Soulié A, Sasportes M
Hum Immunol. 1986 Sep;17(1):30-6. doi: 10.1016/0198-8859(86)90071-6.
Allostimulated T lymphocytes were cloned by micromanipulation and expanded in IL-2 conditioned medium. Three T3+,T4+,T8-, clones called BJ1, BJ4, and BJ37, were extensively studied. The BJ1 cells were able to proliferate and kill the specific target. The BJ4 and BJ37 cells were able to proliferate with the specific restimulator but could not kill even in lectin-dependent cell-mediated cytotoxic assay; however, they acquired the specific cytolytic activity in the 6-day culture when fresh irradiated autologous peripheral blood mononuclear cells as feeder cells were added to the specific irradiated Epstein-Barr virus transformed cell line, in the presence of recombinant IL-2. This observation strongly suggested that the culture conditions could be involved in the differentiation of proliferative clones into cytotoxic T lymphocyte (CTL) clones, by the lymphokines, either present in the IL-2 conditioned medium or secreted by the mixed allogeneic irradiated feeder cells. Moreover, it was shown that the acquisition of the cytolytic function could be blocked by the monoclonal antibody LeoA1, previously described and which recognized the TLiSA1 structure involved in the CTL differentiation.
通过显微操作克隆同种异体刺激的T淋巴细胞，并在白细胞介素-2条件培养基中扩增。对三个T3 +、T4 +、T8 -克隆，即BJ1、BJ4和BJ37进行了广泛研究。BJ1细胞能够增殖并杀伤特异性靶细胞。BJ4和BJ37细胞能够与特异性再刺激物一起增殖，但即使在凝集素依赖性细胞介导的细胞毒性试验中也不能杀伤；然而，当在重组白细胞介素-2存在的情况下，将新鲜照射的自体外周血单核细胞作为饲养细胞添加到特异性照射的爱泼斯坦-巴尔病毒转化细胞系中时，它们在6天培养中获得了特异性溶细胞活性。这一观察结果强烈表明，培养条件可能通过白细胞介素-2条件培养基中存在的或由混合的同种异体照射饲养细胞分泌的淋巴因子，参与增殖性克隆向细胞毒性T淋巴细胞（CTL）克隆的分化。此外，研究表明，溶细胞功能的获得可被先前描述的、识别参与CTL分化的TLiSA1结构的单克隆抗体LeoA1阻断。
