Institute of New Drug Development, China Medical University, No.91 Hsueh-Shih Road, Taichung, 404333, Taiwan.
Division of Neurosurgery, China Medical University Hospital, No.2 Yuh-Der Road, Taichung, 404332, Taiwan.
Food Chem Toxicol. 2022 Jan;159:112761. doi: 10.1016/j.fct.2021.112761. Epub 2021 Dec 8.
Pulmonary inflammation involves complex immune responses in which alveolar macrophages release pro-inflammatory proteins and cytokines. Cardamonin is a spice component that exerts anti-inflammatory and anti-oxidative properties against pulmonary inflammation. Herein, the aim of this research is to investigate the effects of cardamonin on pulmonary inflammation and its mechanism. Pulmonary inflammation in mice was induced by intratracheal administration of PMA. PMA-stimulated acute fibrosis, pulmonary edema, and inflammatory responses were ameliorated by oral administration of cardamonin in vivo. In MH-S alveolar macrophages, PMA-induced pro-inflammatory responses, including iNOS, COX-2, MMP-9 and cytokines expressions were reduced by cardamonin. The anti-oxidative Nrf2/HO-1 axis was also provoked by cardamonin in MH-S alveolar macrophages. In addition, MMP-9 expression induced by PMA is also decreased by the down-stream metabolites of HO-1, indicating that HO-1 expression partially contributes to the anti-inflammatory effect exerted by cardamonin. In this study, cardamonin demonstrates anti-inflammatory and anti-oxidative effects on PMA-induced pulmonary inflammation and activating Nrf2/HO-1 axis in alveolar macrophages. Cardamonin also ameliorates pulmonary inflammation, rapid fibrosis in vivo, suggesting powerful health benefits.
肺部炎症涉及复杂的免疫反应，其中肺泡巨噬细胞释放促炎蛋白和细胞因子。小豆蔻明是一种香料成分，具有抗炎和抗氧化特性，可对抗肺部炎症。本研究旨在探讨小豆蔻明对肺部炎症的作用及其机制。通过气管内给予 PMA 诱导小鼠肺部炎症。体内给予小豆蔻明可改善 PMA 刺激引起的急性纤维化、肺水肿和炎症反应。在 MH-S 肺泡巨噬细胞中，小豆蔻明可降低 PMA 诱导的促炎反应，包括 iNOS、COX-2、MMP-9 和细胞因子的表达。小豆蔻明还可在 MH-S 肺泡巨噬细胞中引发抗氧化 Nrf2/HO-1 轴。此外，HO-1 的下游代谢产物也可降低 PMA 诱导的 MMP-9 表达，表明 HO-1 表达部分有助于小豆蔻明发挥抗炎作用。在这项研究中，小豆蔻明对 PMA 诱导的肺部炎症具有抗炎和抗氧化作用，并在肺泡巨噬细胞中激活 Nrf2/HO-1 轴。小豆蔻明还可改善肺部炎症和体内快速纤维化，提示其具有强大的健康益处。
