苦瓜属藤本植物中的葫芦烷型三萜及其抗炎、细胞毒性和抗糖尿病活性。
Cucurbitane-type triterpenoids from the vines of Momordica charantia and their anti-inflammatory, cytotoxic, and antidiabetic activity.
机构信息
National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, 11201, Taiwan; Department of Biochemical Science and Technology, National Chiayi University, Chiayi, 60004, Taiwan.
National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, 11201, Taiwan; Department of Biochemical Science & Technology, National Taiwan University, Taipei, 10617, Taiwan.
出版信息
Phytochemistry. 2022 Mar;195:113026. doi: 10.1016/j.phytochem.2021.113026. Epub 2021 Dec 7.
Phytochemical investigation of the ethanol extract from wild Momordica charantia vines has resulted in isolation of seven cucurbitane-type triterpenoids, including six undescribed compounds, kuguaovins H‒M, and the known compound, momordicoside K. The structures of the isolated compounds were elucidated on the basis of spectroscopic analyses, including 1D and 2D NMR, and MS experiments. The chemical structure of momordicoside K was determined for the first time by X-ray crystallographic analysis and its absolute configuration assigned. The cytotoxicity against four human tumor cell lines and anti-inflammatory activities on LPS-stimulated RAW264.7 macrophages were evaluated. Of the isolates, kaguaovin L exhibited potential cytotoxicity against MCF-7, HEp-2, Hep-G2, and WiDr cancer cell lines and showed moderate anti-NO production activity. In addition, kuguaovins H and J also showed the stimulatory effect of GLP-1 secretion on the murine intestinal secretin tumor cell line (STC-1).
从野生苦瓜藤的乙醇提取物中进行植物化学研究，分离得到了七种葫芦烷型三萜类化合物，包括六种未描述的化合物，苦瓜甙 H-M，以及已知的化合物苦瓜甙 K。根据光谱分析，包括 1D 和 2D NMR 和 MS 实验，确定了分离化合物的结构。通过 X 射线晶体学分析首次确定了苦瓜甙 K 的化学结构，并确定了其绝对构型。评估了对四种人肿瘤细胞系的细胞毒性和对 LPS 刺激的 RAW264.7 巨噬细胞的抗炎活性。在分离的化合物中，苦瓜甙 L 对 MCF-7、HEp-2、Hep-G2 和 WiDr 癌细胞系表现出潜在的细胞毒性，并显示出中等的抗 NO 产生活性。此外，苦瓜甙 H 和 J 还对小鼠肠促胰素肿瘤细胞系（STC-1）的 GLP-1 分泌表现出刺激作用。
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