Hu Xiao-Yan, Yin Jiao-Jiao, Chao Rong, Zhu Sheng-Dong, Deng Wei, Pan Ming
Department of Pediatric General Medicine, Gansu Maternal and Child Health Hospital, Lanzhou 730050, Gansu Province, China.
Department of Laboratory Medicine, Gansu Maternal and Child Health Hospital, Lanzhou 730050, Gansu Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2021 Dec;29(6):1727-1732. doi: 10.19746/j.cnki.issn.1009-2137.2021.06.006.
To observe the expression of plasma microRNA (miR)-146a and miR-223 in children with acute lymphoblastic leukemia (ALL), so as to analyze the relationship between the two factors and the prognosis of children with ALL.
100 children with ALL treated in the hospital from January 2015 to December 2017 were selected, according to the standard of Chinese Children's Leukemia Group (CCLG)-ALL-2008 program, the children were performed standardized treatment in our hospital according to different risk degree, the follow-up results were obtained, the follow-up time was ≥36 months, and the follow-up time was till to March 2021, the recurrence and mortality of the children were used as prognostic indicators; the baseline data of the children at admission were inquired and recorded, the plasma miR-146a and miR-223 levels were analyzed at admission, and their correlation with the prognosis of children with ALL was analyzed.
During the follow-up period, 4 cases of children died while 18 cases recurred, which means 22(22.00%) children showed the poor prognosis; the plasma miR-146a level of the children in poor prognosis group at admission was higher than those in good prognosis group, while the plasma miR-223 level was lower than those in good prognosis group, the differences showed statistically significantly (P<0.05); the results of regression analysis showed that the over expression of plasma miR-146a and low expression of plasma miR-223 at admission might be associated with poor prognosis in ALL children, and might be a risk factor for poor prognosis in children (P<0.05); the ROC curve showed that the AUC of plasma miR-146a and miR-223 at admission alone or in combination showed the predictive value for the risk of poor prognosis in children with ALL(AUC >0.80); the results of correlation test showed that there was a negative correlation of plasma miR-146a with miR-223 levels at admission (r=-0.239, P=0.016).
Plasma miR-146a is overexpressed and miR-223 is low-expressed in children with ALL, the abnormal expression of the two factors is related to the prognosis of children with ALL.
观察急性淋巴细胞白血病（ALL）患儿血浆微小RNA（miR）-146a和miR-223的表达情况，分析二者与ALL患儿预后的关系。
选取2015年1月至2017年12月在我院治疗的100例ALL患儿，按照中国儿童白血病协作组（CCLG）-ALL-2008方案标准，根据不同危险度在我院进行规范化治疗，获取随访结果，随访时间≥36个月，截至2021年3月，将患儿的复发和死亡情况作为预后指标；查询并记录患儿入院时的基线资料，分析入院时血浆miR-146a和miR-223水平，并分析其与ALL患儿预后的相关性。
随访期间，4例患儿死亡，18例复发，即22例（22.00%）患儿预后不良；预后不良组患儿入院时血浆miR-146a水平高于预后良好组，而血浆miR-223水平低于预后良好组，差异有统计学意义（P<0.05）；回归分析结果显示，入院时血浆miR-146a过表达及血浆miR-223低表达可能与ALL患儿预后不良相关，可能是患儿预后不良的危险因素（P<0.05）；ROC曲线显示，入院时血浆miR-146a和miR-223单独或联合检测对ALL患儿预后不良风险均有预测价值（AUC>0.80）；相关性检验结果显示，入院时血浆miR-
