Guangdong-Macau Traditional Chinese Medicine Technology Industrial Park Development Co., Ltd., Zhuhai 519000, China; Chinese Academy of Sciences Shanghai Institute of Materia Medica, 201210, China.
Jiangxi Key Laboratory of Active Ingredients of Natural Drugs, Yichun University, Yichun 336000, China.
Carbohydr Polym. 2022 Feb 1;277:118877. doi: 10.1016/j.carbpol.2021.118877. Epub 2021 Nov 9.
Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min. The separation was performed on a Cortecs C₁₈ column using acetonitrile (A) and water (B) as the mobile phase with gradient elution. The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode. A comparison with traditional methods was used to determine the validity of the results. The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides. The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs.
在这里，我们报告了一种新的应用，使用乙酰化醛腈衍生物通过液相色谱-电喷雾质谱（UHPLC-ESI-MS），在 14 分钟内实现八种中性糖、两种糖醛酸、一种酮糖和八种糖醇的基线分离和同时检测。分离在 Cortecs C₁₈ 柱上进行，使用乙腈（A）和水（B）作为流动相进行梯度洗脱。通过质谱在选择离子监测（SIM）模式下检测目标成分，采用电喷雾电离（ESI）源在正离子化模式下运行。通过与传统方法进行比较，确定了结果的有效性。UHPLC-ESI-MS 方法用于测定山楂水提取物中游离碳水化合物的定量分析，以及测定多糖中黄精和灵芝单糖。结果表明，该方案是一种全面有效的技术，可用于中药植物多糖的定性和定量分析。
