Department of Obstetrics and Gynaecology, Medical University of Graz, 8036, Graz, Austria; BioTechMed-Graz, Austria.
Department of Obstetrics and Gynaecology, Medical University of Graz, 8036, Graz, Austria; BioTechMed-Graz, Austria.
Placenta. 2022 Jan;117:150-153. doi: 10.1016/j.placenta.2021.12.007. Epub 2021 Dec 3.
Depression is frequent among pregnant women and decision for treatment with antidepressants needs careful consideration of risks for the fetus. Since data regarding fetal antidepressant exposure are rare, we aimed to evaluate transplacental transfer of venlafaxine, a selective norepinephrine reuptake inhibitor.
Ex vivo human placental perfusion experiments were conducted in double closed set-up. Venlafaxine (18.1 ± 2.1 μg/L) was offered in maternal circuit and maternal-to-fetal transfer was monitored over a period of 3h. Venlafaxin and O-desmethylvenlafaxine concentrations were determined by HPLC-MS in maternal and fetal perfusion medium.
We observed maternal-to-fetal transfer of venlafaxine within 5 min perfusion. The concentration equilibrium was approximated between maternal (7.5 ± 0.5 μg/L) and fetal (6.5 ± 0.6 μg/L) compartment at time point 180 min, which corresponds to a fetal-maternal (FM) ratio of 0.89.
Our results are comparable with in vivo data from an observational study which emphasizes that the ex vivo placental perfusion model is suitable for systematic evaluation of fetal antidepressant exposure.
抑郁症在孕妇中很常见，因此需要仔细考虑治疗用抗抑郁药对胎儿的风险。由于关于胎儿接触抗抑郁药的数据很少，我们旨在评估文拉法辛（一种选择性去甲肾上腺素再摄取抑制剂）的胎盘转移。
在双封闭系统中进行体外人胎盘灌注实验。在母体回路中提供文拉法辛（18.1±2.1μg/L），并在 3 小时的时间内监测母体到胎儿的转移。通过 HPLC-MS 在母体和胎儿灌注介质中测定文拉法辛和 O-去甲文拉法辛的浓度。
我们观察到文拉法辛在灌注 5 分钟内即可从母体转移至胎儿。在 180 分钟时，母体内（7.5±0.5μg/L）和胎儿内（6.5±0.6μg/L）达到浓度平衡，这对应于胎儿-母体（FM）比值为 0.89。
我们的结果与来自观察性研究的体内数据相当，这强调了体外胎盘灌注模型适合系统评估胎儿接触抗抑郁药的情况。
