Zwilling B S, Aldrich W A, Rinehart J J
Cancer Res. 1986 Nov;46(11):5667-70.
Culture of spleen cells from strain 2 guinea pigs with Jurkat interleukin-2 (IL-2) resulted in the induction of lymphokine-activated killer (LAK) cells. Maximum LAK activity was induced using 5000 pmol of IL-2. Incubation of spleen cells with IL-2 for as little as 8 h resulted in detectable LAK activity. LAK cell activity was transient and could be stimulated by adding back IL-2. LAK cell activity was enriched in a 1.085 single-step percoll gradient. Admixture of guinea pig LAK cells with the line 10 hepatoma prior to intradermal injection resulted in inhibition of tumor growth. Systemic passive transfer of LAK cells together with concurrent IL-2 resulted in a significant inhibition of metastatic tumor growth.
用白细胞介素-2（IL-2）培养2系豚鼠的脾细胞可诱导产生淋巴因子激活的杀伤（LAK）细胞。使用5000皮摩尔的IL-2可诱导出最大的LAK活性。脾细胞与IL-2孵育仅8小时就可检测到LAK活性。LAK细胞活性是短暂的，可通过重新添加IL-2来刺激。LAK细胞活性在1.085的单步Percoll梯度中得到富集。豚鼠LAK细胞与10号线肝癌细胞在皮内注射前混合可抑制肿瘤生长。LAK细胞与同时使用的IL-2进行全身被动转移可显著抑制转移性肿瘤的生长。
